| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OLIG3 |
| Uniprot No | Q7RTU3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-272 aa |
| 活性数据 | MNSDSSSVSS RASSPDMDEM YLRDHHHRHH HHQESRLNSV SSTQGDMMQK MPGESLSRAG AKAAGESSKY KIKKQLSEQD LQQLRLKING RERKRMHDLN LAMDGLREVM PYAHGPSVRK LSKIATLLLA RNYILMLTSS LEEMKRLVGE IYGGHHSAFH CGTVGHSAGH PAHAANSVHP VHPILGGALS SGNASSPLSA ASLPAIGTIR PPHSLLKAPS TPPALQLGSG FQHWAGLPCP CTICQMPPPP HLSALSTANM ARLSAESKDL LK |
| 分子量 | 29.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为与OLIG3蛋白功能相关的3篇代表性文献的简化摘要概述(注：部分内容基于假设性场景整理)：
1. **文献名称**：*Olig3 regulates the regionalization of prethalamic nuclei during mouse brain development*
**作者**：K. Kitamura 等
**摘要**：研究发现OLIG3作为转录因子调控小鼠胚胎期丘脑前区的神经前体细胞分化，通过遗传学手段证实其缺失会导致特定神经核团的发育异常，影响神经元迁移路径。
2. **文献名称**：*The expression dynamics of Olig3 in dorsal spinal cord progenitor cells*
**作者**：R.M. Tuttle 等
**摘要**：该文通过免疫组化及原位杂交技术解析OLIG3在小鼠脊髓背侧祖细胞中的动态表达模式，揭示其与Atoh1等基因的协同作用，参与感觉神经元的特化过程。
3. **文献名称**：*Olig3 deficiency alters nociceptive sensory neuron connectivity in the spinal cord*
**作者**：L. Tjian 等
**摘要**：利用Olig3敲除小鼠模型，研究发现其缺失导致脊髓背角伤害性感觉神经突触连接异常，提示OLIG3可能参与痛觉信号传导通路的早期建立。
**说明**：OLIG3研究相对较少且集中在神经发育领域，上述主题内容基于真实研究方向模拟。实际应用中建议通过PubMed/Google Scholar以"OLIG3 protein function"为关键词检索近年研究获取具体文献。
Oligodendrocyte transcription factor 3 (OLIG3) is a member of the OLIG family of basic helix-loop-helix (bHLH) transcription factors, primarily recognized for its role in embryonic development and neural patterning. Initially identified through homology with OLIG1 and OLIG2. OLIG3 is distinguished by its restricted expression patterns and specific regulatory functions during neurogenesis. In vertebrates, OLIG3 is transiently expressed in discrete regions of the developing nervous system, notably in the dorsal neural tube, where it participates in the segmentation and specification of neuronal subtypes. Studies in mice suggest that OLIG3 is critical for defining progenitor cell identities in the rhombic lip, a key structure in hindbrain development, and influences the formation of precerebellar nuclei and sensory relay neurons.
Unlike OLIG1/2. which are closely associated with oligodendrocyte differentiation, OLIG3's functions extend to broader developmental contexts, including boundary formation between distinct neuronal domains and suppression of alternative cell fates. Its activity is tightly regulated by signaling pathways such as Wnt and Sonic Hedgehog (Shh), underscoring its integration into complex morphogenetic networks. Recombinant human OLIG3 protein is typically produced using bacterial or mammalian expression systems, enabling in vitro studies to dissect its DNA-binding properties, dimerization partners (e.g., E proteins), and transcriptional targets. Current research focuses on its potential roles in neurodevelopmental disorders and its interplay with other bHLH factors in maintaining neural progenitor pools. Despite progress, OLIG3's post-embryonic functions and mechanistic details remain incompletely characterized, warranting further exploration.
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