| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR10AD1 |
| Uniprot No | Q8NGE0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 0 |
| 活性数据 | MLRNGSIVTEFILVGFQQSSTSTRALLFALFLALYSLTMAMNGLIIFITSWTDPKLNSPMYFFLGHLSLLDVCFITTTIPQMLIHLVVRDHIVSFVCCMTQMYFVFCVGVAECILLAFMAYDRYVAICYPLNYVPIISQKVCVRLVGTAWFFGLINGIFLEYISFREPFRRDNHIESFFCEAPIVIGLSCGDPQFSLWAIFADAIVVILSPMVLTVTSYVHILATILSKASSSGRGKTFSTCASHLTVVIFLYTSAMFSYMNPHSTHGPDKDKPFSLLYTIITPMCNPIIYSFRNKEIKEAMVRALGRTRLAQPQSV |
| 分子量 | 35.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR10AD1蛋白的参考文献示例(注：部分文献为假设性描述，建议通过学术数据库核实)：
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1. **"Deorphanization of human olfactory receptor OR10AD1 through heterologous expression"**
*Mainland et al. (2017)*
摘要：本研究在HEK293细胞中成功表达了重组OR10AD1蛋白，并通过钙离子成像筛选其配体，确定其响应特定挥发性化合物的功能。
2. **"OR10AD1 expression in pulmonary cells and its role in inflammatory signaling"**
*Smith et al. (2020)*
摘要：探讨了OR10AD1在呼吸道细胞中的重组表达，发现其与脂质配体结合后可调节炎症因子释放，提示非嗅觉功能的潜在机制。
3. **"Cryo-EM structure of human olfactory receptor OR10AD1-Gα complex"**
*Zhang et al. (2022)*
摘要：利用昆虫细胞系统表达并纯化OR10AD1蛋白，通过冷冻电镜解析其与G蛋白偶联的复合物结构，揭示信号转导的结构基础。
4. **"High-throughput screening of odorant receptors using nanodisc-embedded OR10AD1"**
*Lee & Park (2021)*
摘要：将重组OR10AD1嵌入纳米盘以模拟膜环境，开发高通量筛选方法，鉴定其对环境气味分子的特异性响应。
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**说明**：以上文献为模拟示例，实际研究中OR10AD1相关文献可能较少，建议通过 **PubMed、Google Scholar** 或 **Web of Science** 以最新关键词检索，并注意确认基因命名变体(如OR10AD1是否曾用其他名称)。
OR10AD1 is a human olfactory receptor belonging to the class A G protein-coupled receptor (GPCR) superfamily. As part of the olfactory receptor (OR) gene cluster on chromosome 1. it plays a critical role in odorant detection and signal transduction, converting chemical stimuli into neural signals. Olfactory receptors like OR10AD1 are transmembrane proteins characterized by seven α-helical domains, with extracellular loops involved in ligand binding. Though primarily expressed in olfactory sensory neurons, some studies suggest ectopic expression in non-olfactory tissues, though its physiological roles there remain unclear.
Recombinant OR10AD1 protein is synthesized using heterologous expression systems (e.g., HEK293. insect cells) with tags like FLAG or GFP to facilitate purification and visualization. Challenges in recombinant production include low natural abundance, membrane protein instability, and the need for lipid bilayer mimetics to maintain structural integrity. Its study is crucial for decoding odorant specificity patterns and understanding receptor-ligand interactions, which may have applications in biomedicine (e.g., targeted drug delivery) and synthetic biology. Recent advances in cryo-EM and structural biology have enabled deeper insights into its activation mechanisms, though its precise endogenous ligands remain to be fully characterized.
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