| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR11L1 |
| Uniprot No | Q8NGX0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-322 aa |
| 活性数据 | MEPQNTSTVTNFQLLGFQNLLEWQALLFVIFLLIYCLTIIGNVVIITVVSQGLRLHSPMY MFLQHLSFLEVWYTSTTVPLLLANLLSWGQAISFSACMAQLYFFVFLGATECFLLAFMAY DRYLAICSPLRYPFLMHRGLCARLVVVSWCTGVSTGFLPSLMISRLDFCGRNQINHFFCD LPPLMQLSCSRVYITEVTIFILSIAVLCICFFLTLGPYVFIVSSILRIPSTSGRRKTFST CGSHLAVVTLYYGTMISMYVCPSPHLLPEINKIISVFYTVVTPLLNPVIYSLRNKDFKEA VRKVMRRKCGILWSTSKRKFLY |
| 分子量 | 36.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于学术文献结构模拟生成的3篇关于OR11L1重组蛋白的参考文献示例(注：实际文献可能需要通过PubMed/Google Scholar等平台验证，以下内容为示例)：
---
1. **文献名称**: *Functional Expression of Olfactory Receptor OR11L1 in HEK293 Cells for Ligand Screening*
**作者**: Sanchez, L., et al.
**摘要**: 本研究成功构建了OR11L1重组蛋白的哺乳动物表达系统(HEK293细胞)，通过荧光钙离子成像技术筛选出2种可能激活该受体的挥发性化合物，揭示了其在非嗅觉组织中的潜在生理功能。
2. **文献名称**: *Cryo-EM Structure Determination of Human OR11L1 in Complex with a Synthetic Odorant*
**作者**: Kim, J., & Müller, C.E.
**摘要**: 利用昆虫细胞表达系统获得高纯度OR11L1重组蛋白，结合冷冻电镜技术首次解析其三维结构，发现其配体结合口袋的独特疏水性特征，为嗅觉受体药物开发提供结构基础。
3. **文献名称**: *OR11L1-Mediated Signal Transduction in Airway Epithelial Cells via Recombinant Protein Assays*
**作者**: Patel, R., et al.
**摘要**: 通过大肠杆菌重组表达OR11L1胞外域蛋白，证明其与呼吸道炎症相关分子存在相互作用，提示该受体可能在免疫应答中发挥新型功能，挑战了嗅觉受体仅参与嗅觉的传统认知。
---
**建议查询策略**：
1. 在PubMed中搜索 **"OR11L1 recombinant expression"** 或 **"OR11L1 structural analysis"**
2. 查找嗅觉受体通用研究(如OR2T1/OR5A1等同家族受体)，类比其重组方法
3. 关注《ACS Chemical Biology》《Journal of Structural Biology》等期刊近年论文
4. 联系嗅觉受体研究领域实验室(如R. Matsunami团队)获取预印本资料
真实文献可能存在研究空白，建议结合 **UniProt数据库(ID: Q8NGF2)** 的参考文献模块获取已验证信息。
**Recombinant human OR11L1 protein** belongs to the olfactory receptor (OR) family, a class of G protein-coupled receptors (GPCRs) predominantly involved in odorant detection. OR11L1. encoded by the *OR11L1* gene, is categorized within the OR subfamily 11. though its specific ligands and physiological roles remain largely uncharacterized. Olfactory receptors are typically expressed in olfactory sensory neurons, where they bind odorant molecules to initiate signal transduction. However, emerging studies suggest their expression in non-olfactory tissues (e.g., brain, testes), hinting at broader functions in cellular processes or disease pathways.
Producing recombinant OR11L1 poses challenges due to the inherent difficulty in expressing GPCRs in heterologous systems (e.g., bacterial, yeast, or mammalian cells), often requiring optimized vectors, fusion tags, or lipid-based stabilization. Recombinant OR11L1 enables structural studies (e.g., crystallography, cryo-EM) and high-throughput screening for ligand identification, aiding in decoding its biological relevance. Additionally, it serves as a tool to explore potential roles in diseases like cancer or neurological disorders, where ectopic OR expression has been reported.
Despite limited functional data, OR11L1’s conservation across mammals underscores its evolutionary significance. Ongoing research aims to unravel its ligand specificity, signaling mechanisms, and extranasal roles, bridging gaps in understanding OR diversity and therapeutic applications.
×
