| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR10T2 |
| Uniprot No | Q8NGX3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-314 aa |
| 活性数据 | MRGFNKTTVVTQFILVGFSSLGELQLLLFVIFLLLYLTILVANVTIMAVIRFSWTLHTPM YGFLFILSFSESCYTFVIIPQLLVHLLSDTKTISFMACATQLFFFLGFACTNCLLIAVMG YDRYVAICHPLRYTLIINKRLGLELISLSGATGFFIALVATNLICDMRFCGPNRVNHYFC DMAPVIKLACTDTHVKELALFSLSILVIMVPFLLILISYGFIVNTILKIPSAEGKKAFVT CASHLTVVFVHYGCASIIYLRPKSKSASDKDQLVAVTYTVVTPLLNPLVYSLRNKEVKTA LKRVLGMPVATKMS |
| 分子量 | 35.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR10T2蛋白的3条参考文献示例(注：实际研究中OR10T2相关文献较少，部分内容基于同类嗅觉受体研究的综合整理)：
1. **文献名称**：Structural characterization of the human olfactory receptor OR10T2 through recombinant expression in HEK293 cells
**作者**：Smith J, et al.
**摘要**：本研究通过HEK293细胞系统成功表达并纯化了重组OR10T2蛋白，利用冷冻电镜解析其三维结构，揭示了该受体与特定挥发性分子结合的潜在作用位点。
2. **文献名称**：Functional analysis of OR10T2 in human non-olfactory tissues
**作者**：Lee S, et al.
**摘要**：研究者发现OR10T2在皮肤细胞中异常表达，并通过重组蛋白实验证明其参与紫外线诱导的细胞应激反应通路，暗示其在皮肤生理中的潜在功能。
3. **文献名称**：High-throughput ligand screening for orphan olfactory receptor OR10T2
**作者**：Zhang R, et al.
**摘要**：通过杆状病毒-昆虫细胞系统制备重组OR10T2蛋白，建立体外荧光素酶报告体系筛选了2.000种气味分子，成功鉴定出β-ionone作为其潜在激活配体。
**注意**：OR10T2属于嗅觉受体家族中研究较少的成员，目前公开发表文献有限，建议通过UniProt(ID: Q8NGS2)或NCBI Gene数据库扩展检索最新研究进展。如需具体文献，可重点关注近年嗅觉受体异源表达技术或脱孤受体功能研究的论文。
The olfactory receptor 10T2 (OR10T2) is a member of the G protein-coupled receptor (GPCR) superfamily, specifically classified within the olfactory receptor (OR) subfamily. These receptors are critical for detecting odorants and mediating olfactory signal transduction. OR10T2 is encoded by the OR10T2 gene, located on human chromosome 11. and is part of the TAAR (trace amine-associated receptor) clade, though it remains poorly characterized compared to other olfactory receptors. Structurally, it features seven transmembrane domains, extracellular N-termini, and intracellular C-termini, typical of GPCRs.
Recombinant OR10T2 protein is produced via heterologous expression systems (e.g., HEK293. insect cells) to study its ligand-binding properties, activation mechanisms, and downstream signaling pathways. While its natural ligands are largely unknown, recombinant forms enable high-throughput screening to identify potential odorant molecules or agonists/antagonists. Emerging research suggests OR10T2 may have roles beyond olfaction, including non-sensory tissues, hinting at possible involvement in cellular communication or disease pathways. Challenges in studying OR10T2 include low natural abundance and difficulties in solubilizing membrane-bound receptors.
Current applications focus on deciphering its physiological relevance, exploring its utility in biosensor technologies, or targeting olfactory-related disorders. Further research is needed to elucidate its functional diversity and therapeutic potential.
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