| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR10Z1 |
| Uniprot No | Q8NGY1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-313 aa |
| 活性数据 | MGQTNVTSWRDFVFLGFSSSGELQLLLFALFLSLYLVTLTSNVFIIIAIRLDSHLHTPMY LFLSFLSFSETCYTLGIIPRMLSGLAGGDQAISYVGCAAQMFFSASWACTNCFLLAAMGF DRYVAICAPLHYASHMNPTLCAQLVITSFLTGYLFGLGMTLVIFHLSFCSSHEIQHFFCD TPPVLSLACGDTGPSELRIFILSLLVLLVSFFFITISYAYILAAILRIPSAEGQKKAFST CASHLTVVIIHYGCASFVYLRPKASYSLERDQLIAMTYTVVTPLLNPIVYSLRNRAIQTA LRNAFRGRLLGKG |
| 分子量 | 34.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR10Z1蛋白的模拟参考文献示例，基于常见研究方向的合理推测，供参考：
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### 1. **《异源表达与纯化重组人OR10Z1嗅觉受体的优化策略》**
**作者：** Zhang L., et al.
**摘要：**
本研究通过哺乳动物细胞表达系统(HEK293)成功实现了OR10Z1蛋白的可溶性重组表达。采用亲和层析与脂质体重组技术纯化蛋白，并通过Western blot和质谱验证其完整性。该工作为后续研究OR10Z1的配体识别提供了高纯度蛋白样本。
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### 2. **《OR10Z1的配体筛选及信号转导机制研究》**
**作者：** Müller F., et al.
**摘要：**
通过高通量筛选结合钙离子流检测，鉴定出OR10Z1对特定挥发性酯类分子的激活反应。结构模拟显示，该受体第3跨膜结构域的疏水口袋是配体结合的关键区域，提示其可能的生理功能与特定气味分子识别相关。
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### 3. **《非嗅觉组织中OR10Z1的表达及其在炎症调控中的作用》**
**作者：** Chen Y., et al.
**摘要：**
在结肠癌细胞系中发现OR10Z1异常高表达，RNA干扰沉默后炎症因子(如IL-6、TNF-α)显著下调。研究推测OR10Z1可能通过MAPK通路参与炎症反应，为探索其作为治疗靶点提供依据。
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**注：** 上述文献为模拟示例，实际研究请通过学术数据库(如PubMed、Web of Science)以“OR10Z1”、“recombinant expression”、“olfactory receptor”等关键词检索。
**Background of Recombinant Human OR10Z1 Protein**
The recombinant human OR10Z1 (Olfactory Receptor Family 10 Subfamily Z Member 1) protein belongs to the olfactory receptor (OR) family, a subset of G protein-coupled receptors (GPCRs) crucial for detecting odorant molecules in the olfactory system. ORs are characterized by seven transmembrane domains and extracellular ligand-binding regions, enabling selective interaction with specific chemical compounds. While most ORs are expressed in olfactory sensory neurons, emerging studies suggest their presence in non-olfactory tissues (e.g., testes, lungs, and immune cells), hinting at broader physiological roles beyond smell, such as cellular signaling or disease pathways.
OR10Z1 remains relatively understudied, with its exact endogenous ligands and activation mechanisms unclear. Recombinant OR10Z1 is typically produced in heterologous systems (e.g., mammalian or insect cells) using genetic engineering, enabling high-purity protein isolation for functional studies. This recombinant form facilitates research into its structure, ligand specificity, and downstream signaling, often via calcium flux or cAMP assays.
Interest in OR10Z1 extends to potential therapeutic applications, as aberrant OR expression has been linked to cancers and metabolic disorders. However, challenges persist due to ORs’ complex ligand interactions and low natural expression levels. Continued exploration of OR10Z1 may uncover novel biomarkers or drug targets, bridging gaps in sensory biology and disease mechanisms.
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