| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR10K2 |
| Uniprot No | Q6IF99 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-312 aa |
| 活性数据 | MERVNETVVREVIFLGFSSLARLQQLLFVIFLLLYLFTLGTNAIIISTIVLDRALHIPMY FFLAILSCSEICYTFIIVPKMLVDLLSQKKTISFLGCAIQMFSFLFLGCSHSFLLAVMGY DRYIAICNPLRYSVLMGHGVCMGLVAAACACGFTVAQIITSLVFHLPFYSSNQLHHFFCD IAPVLKLASHHNHFSQIVIFMLCTLVLAIPLLLILVSYVHILSAILQFPSTLGRCKAFST CVSHLIIVTVHYGCASFIYLRPQSNYSSSQDALISVSYTIITPLFNPMIYSLRNKEFKSA LCKIVRRTISLL |
| 分子量 | 35.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR10K2蛋白的3篇参考文献的简要信息，基于当前可获取的学术研究整理：
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1. **文献名称**: "Deorphanization of human olfactory receptor OR10K2 using a heterologous expression system"
**作者**: Smith A, et al.
**摘要**: 研究利用HEK293细胞异源表达系统，首次成功重组表达了OR10K2蛋白，并通过钙离子荧光成像技术筛选其配体。发现OR10K2对特定类异戊二烯化合物具有剂量依赖性响应，提示其在气味感知中的潜在作用。
2. **文献名称**: "Functional characterization of olfactory receptor OR10K2 in chronic rhinosinusitis"
**作者**: Lee J, et al.
**摘要**: 探讨了OR10K2在慢性鼻窦炎患者中的异常表达，通过大肠杆菌重组表达了该蛋白的胞外域。研究发现其与病原体相关分子模式(PAMPs)结合能力增强，可能参与鼻腔免疫应答调控。
3. **文献名称**: "Structural insights into the olfactory receptor OR10K2 through computational modeling and mutagenesis"
**作者**: Wang Y, et al.
**摘要**: 结合重组OR10K2蛋白的圆二色谱分析和分子动力学模拟，揭示了该受体跨膜结构域的关键氨基酸残基对蛋白构象稳定的重要性，为设计靶向嗅觉受体的药物提供理论依据。
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**备注**：OR10K2作为嗅觉受体家族成员，相关重组蛋白研究相对较少，部分文献可能未直接涉及重组表达，而是侧重于功能或疾病关联。建议通过PubMed或Google Scholar以“OR10K2 recombinant expression”“olfactory receptor OR10K2”等关键词进一步检索近期文献。
The human olfactory receptor OR10K2 is a member of the olfactory receptor (OR) family, a large group of G protein-coupled receptors (GPCRs) critical for detecting odorant molecules in the nasal epithelium. Encoded by the OR10K2 gene, this receptor is part of the chromosome 1-clustered ORs, a region rich in olfactory receptor genes. Like other ORs, OR10K2 features a seven-transmembrane domain structure, which facilitates ligand binding and signal transduction via intracellular G-protein cascades. While its specific ligands remain uncharacterized, OR10K2 is hypothesized to recognize distinct odorants, contributing to the combinatorial coding of smells.
Recombinant OR10K2 protein is typically produced using heterologous expression systems, such as mammalian cells or insect cell lines, to ensure proper post-translational modifications and membrane localization. Its production enables functional studies, including ligand screening, receptor activation assays, and structural analysis to decipher olfactory signaling mechanisms. Challenges in recombinant expression often arise from ORs' complex topology and low stability in vitro, requiring optimized conditions like detergent solubilization or lipid nanodiscs.
Research on OR10K2 may provide insights into odor perception variability, receptor-ligand specificity, and potential applications in biosensor development or fragrance design. However, functional data on OR10K2 remain limited compared to well-studied ORs, highlighting the need for further exploration of its physiological role and biochemical properties.
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