| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR1A2 |
| Uniprot No | Q9Y585 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 0 |
| 活性数据 | MKKENQSFNLDFILLGVTSQQEQNNVFFVIFLCIYPITLTGNLLIILAICADIRLHNPMYFLLANLSLVDIIFSSVTIPKVLANHLLGSKFISFGGCLMQMYFMIALAKADSYTLAAMAYDRAVAISCPLHYTTIMSPRSCILLIAGSWVIGNTSALPHTLLTASLSFCGNQEVANFYCDIMPLLKLSCSDVHFNVKMMYLGVGVFSLPLLCIIVSYVQVFSTVFQVPSTKSLFKAFCTCGSHLTVVFLYYGTTMGMYFRPLTSYSPKDAVITVMYVAVTPALNPFIYSLRNWDMKAALQKLFSKRISS |
| 分子量 | 34.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR1A2蛋白的3篇代表性文献的模拟概述(基于公开研究主题推测，实际文献需通过数据库验证)：
1. **文献名称**："Functional expression of human olfactory receptor OR1A2 in HEK293 cells reveals ligand specificity"
**作者**：Smith J, et al.
**摘要**：研究成功在HEK293细胞中表达重组OR1A2蛋白，通过钙流实验鉴定其配体特异性，发现其对柑橘类挥发物(如柠檬烯)具有显著响应，为嗅觉受体体外功能研究提供方法参考。
2. **文献名称**："Structural insights into the human olfactory receptor OR1A2 through computational modeling"
**作者**：Li X, Zhang Y.
**摘要**：采用分子动力学模拟和同源建模，预测OR1A2的三维结构及配体结合位点，揭示其与挥发性有机化合物结合的氨基酸残基特征，为嗅觉受体的药物开发奠定基础。
3. **文献名称**："OR1A2 expression in non-olfactory tissues and its role in metabolic regulation"
**作者**：Wang Q, et al.
**摘要**：发现OR1A2在肝脏和脂肪组织中低水平表达，重组蛋白实验表明其参与脂肪酸代谢调控，暗示嗅觉受体在嗅觉以外的生理功能中的潜在作用。
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**说明**：以上文献内容为模拟概括，OR1A2属嗅觉受体家族(ORs)，研究多集中于配体筛选、结构解析及非嗅觉功能。建议通过PubMed/Google Scholar以“OR1A2”、“recombinant expression”、“olfactory receptor”等关键词检索真实文献。
**Background of Recombinant Human OR1A2 Protein**
The human olfactory receptor OR1A2. a member of the olfactory receptor (OR) family, is a G protein-coupled receptor (GPCR) primarily associated with the detection of odorants. ORs are encoded by the largest gene family in the human genome, playing critical roles in sensing chemical stimuli and initiating olfactory signaling. OR1A2 is expressed in olfactory epithelial tissues, though emerging studies suggest its potential presence in non-olfactory tissues, such as the central nervous system, liver, or reproductive organs, hinting at broader physiological roles beyond smell.
Recombinant OR1A2 protein is artificially produced using heterologous expression systems (e.g., mammalian cells or bacteria) to study its structure, ligand specificity, and signaling mechanisms. OR1A2 is known to interact with specific volatile compounds, including certain citrus or mint-derived molecules, influencing sensory perception and potential applications in flavor/fragrance industries.
Research on recombinant OR1A2 focuses on deciphering its activation pathways, ligand-binding dynamics, and polymorphisms linked to odor sensitivity variations. Its recombinant form enables high-throughput screening for odorant discovery or therapeutic candidates targeting OR-related disorders. Additionally, structural studies using recombinant OR1A2 contribute to understanding GPCR dynamics, aiding drug development for non-olfactory diseases. Despite challenges in functional expression due to ORs’ complexity, advances in protein engineering continue to unlock its biomedical and industrial potential.
This protein serves as a model for exploring GPCR diversity and chemosensory interactions, bridging gaps between molecular biology and sensory neuroscience.
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