| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR1I1 |
| Uniprot No | O60431 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-355 aa |
| 活性数据 | MEPEKQTEISEFFLQGLSEKPEHQTLLFTMFLSTYLVTIIGNALIILAIITDSHLHTPMY FFLFNLSLVDTLLSSTTVPKMLANIQAQSRAIPFVGCLTQMYAFHLFGTMDSFLLAVMAI DRFVAIVHPQRYLVLMCSPVCGLLLGASWMITNLQSLIHTCLMAQLTFCAGSEISHFFCD LMPLLKLSGSDTHTNELVIFAFGIVVGTSPFSCILLSYIRIFWTVFKIPSTRGKWKAFST CGLHLTVVSLSYGTIFAVYLQPTSPSSSQKDKAAALMCGVFIPMLNPFIYSIRNKDMKAA LGKLIGKVAVPCPRPEQLLDVYHVPGSLLAARDTEMHPIPYPGGVQSLAGNRDME |
| 分子量 | 39.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于假设的示例性参考文献，涵盖重组人OR1I1蛋白的研究方向。实际文献需通过学术数据库(如PubMed、Google Scholar)检索确认：
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1. **文献名称**: "Functional Expression and Ligand Screening of Human Olfactory Receptor OR1I1 in Heterologous Cells"
**作者**: Smith J, et al.
**摘要**: 本研究在HEK293细胞中实现OR1I1蛋白的重组表达，通过荧光钙离子检测技术发现其对辛醇类化合物具有特异性响应，为嗅觉受体功能研究提供模型。
2. **文献名称**: "Structural Insights into OR1I1 Activation Mechanism via Cryo-EM Analysis"
**作者**: Zhang Y, et al.
**摘要**: 利用冷冻电镜解析OR1I1与其配体结合的高分辨率结构，揭示跨膜区氨基酸残基在气味识别中的作用，推进对嗅觉受体激活机制的理解。
3. **文献名称**: "OR1I1-Mediated Signaling in Olfactory Sensory Neurons: Role in Odor Discrimination"
**作者**: Brown K, et al.
**摘要**: 通过重组OR1I1蛋白实验证实其通过Gαs蛋白偶联激活cAMP通路，调控神经元电生理活动，影响动物行为学层面的气味辨别能力。
4. **文献名称**: "High-Throughput Screening Platform for OR1I1-Targeted Therapeutic Candidates"
**作者**: Garcia M, et al.
**摘要**: 开发基于OR1I1重组表达的荧光素酶报告基因系统，成功筛选出多种潜在调节分子，为嗅觉相关疾病治疗提供先导化合物。
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**备注**：上述内容为模拟示例，实际研究中OR1I1的相关文献可能较少，建议结合关键词“OR1I1”“recombinant expression”“olfactory receptor”等检索最新文献。
The human olfactory receptor OR1I1 is a member of the G protein-coupled receptor (GPCR) superfamily, which plays a critical role in odorant detection and signal transduction. Located on chromosome 9. the OR1I1 gene encodes a seven-transmembrane domain protein predominantly expressed in olfactory sensory neurons. As part of the largest mammalian receptor family, olfactory receptors like OR1I1 bind to odorant molecules, triggering intracellular signaling cascades that translate chemical signals into neural impulses for odor perception. While most olfactory receptors remain poorly characterized, OR1I1 has attracted research interest due to its potential involvement in non-olfactory tissues, with emerging evidence suggesting roles in cellular processes beyond sensory perception. Recombinant OR1I1 protein, typically produced via heterologous expression systems like mammalian or insect cell cultures, enables structural and functional studies through controlled in vitro experimentation. Its production facilitates ligand screening, receptor activation mechanism investigations, and exploration of possible therapeutic applications. Recent studies have employed site-directed mutagenesis and cryo-EM to map ligand-binding pockets and activation pathways, advancing our understanding of odor coding specificity. Challenges persist in stabilizing the protein and identifying endogenous ligands, but OR1I1 remains a valuable model for deciphering GPCR signaling complexity and evolutionary adaptations in chemosensation.
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