| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR2K2 |
| Uniprot No | Q8NGT1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-345 aa |
| 活性数据 | MNITLYSFLYVLRLNEGNSTGRNIDERKKMQGENFTIWSIFFLEGFSQYPGLEVVLFVFS LVMYLTTLLGNSTLILITILDSRLKTPMYLFLGNLSFMDICYTSASVPTLLVNLLSSQKT IIFSGCAVQMYLSLAMGSTECVLLAVMAYDRYVAICNPLRYSIIMNRCVCARMATVSWVT GCLTALLETSFALQIPLCGNLIDHFTCEILAVLKLACTSSLLMNTIMLVVSILLLPIPML LVCISYIFILSTILRITSAEGRNKAFSTCGAHLTVVILYYGAALSMYLKPSSSNAQKIDK IISLLYGVLTPMLNPIIYSLRNKEVKDAMKKLLGKITLHQTHEHL |
| 分子量 | 35.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR2K2蛋白的假设性参考文献示例(仅供格式参考，非真实文献)：
1. **文献名称**: "Heterologous expression and functional characterization of human olfactory receptor OR2K2"
**作者**: Smith J, Doe A, Lee C
**摘要**: 本研究成功在HEK293细胞中重组表达了OR2K2蛋白，并利用钙信号检测技术证实其特异性响应β-紫罗兰酮等气味分子，揭示了其G蛋白偶联信号机制。
2. **文献名称**: "Structural insights into OR2K2 ligand-binding specificity using molecular docking"
**作者**: Chen L, Wang H, Kim M
**摘要**: 通过分子对接和位点突变分析，确定了OR2K2蛋白的配体结合口袋关键残基，预测其选择性结合酮类气味化合物的结构基础。
3. **文献名称**: "OR2K2 expression in non-olfactory tissues: Implications in metabolic regulation"
**作者**: Gonzalez R, Patel S, Zhang Y
**摘要**: 研究发现OR2K2在肝脏组织中低水平表达，并通过体外重组蛋白实验证明其可能参与调节脂代谢相关信号通路。
4. **文献名称**: "A high-throughput screening platform for OR2K2 agonists/antagonists"
**作者**: Müller T, Fischer B, Schmidt D
**摘要**: 开发了基于重组OR2K2蛋白的高通量筛选系统，筛选出小分子库中多个潜在配体，为嗅觉受体功能研究提供新工具。
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**注意**：以上文献为虚构示例，实际研究中请通过学术数据库(如PubMed、Google Scholar)检索真实文献。若需具体文献支持，建议结合最新研究并使用关键词“OR2K2”、“olfactory receptor expression”或“GPCR heterologous expression”进行查询。
**Background of Human Olfactory Receptor OR2K2**
Olfactory receptor 2K2 (OR2K2), a member of the olfactory receptor (OR) family, is a G protein-coupled receptor (GPCR) involved in detecting odorant molecules. ORs, encoded by the largest gene family in vertebrates, mediate the sense of smell by binding to volatile chemicals and triggering neuronal signaling. OR2K2 belongs to the OR2K subfamily and is expressed predominantly in olfactory sensory neurons within the olfactory epithelium. Though its specific ligands remain poorly characterized, OR2K2 is hypothesized to recognize distinct odorants, contributing to the broad spectrum of human olfactory perception.
OR genes, including *OR2K2*, are located across multiple chromosomes and often exhibit significant genetic diversity due to single-nucleotide polymorphisms (SNPs), leading to interindividual variations in odor sensitivity. Recent studies suggest ectopic expression of OR2K2 in non-olfactory tissues, such as the brain, heart, and kidneys, hinting at potential roles beyond olfaction, including cellular homeostasis or disease pathways. For instance, OR activation in non-olfactory contexts has been linked to processes like apoptosis regulation and metabolic signaling.
Despite its biological relevance, OR2K2 remains understudied compared to other GPCRs. Challenges include low receptor expression in heterologous systems and limited ligand identification methods. Advances in *deorphanization* techniques (e.g., high-throughput screening) and structural biology may soon clarify OR2K2’s functional mechanisms and physiological impact. Understanding OR2K2 could also inform therapeutic strategies, as olfactory receptors are increasingly implicated in diseases such as neurodegeneration and cancer.
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