| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR4A47 |
| Uniprot No | Q6IF82 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-309 aa |
| 活性数据 | MEPRKNVTDFVLLGFTQNPKEQKVLFVMFLLFYILTMVGNLLIVVTVTVSETLGSPMYFF LAGLSFIDIIYSSSISPRLISGLFFGNNSISFQSCMAQLFIEHIFGGSEVFLLLVMAYDC YVAICKPLHYLVIMRQWVCVVLLVVSWVGGFLHSVFQLSIIYGLPFCGPNVIDHFFCDMY PLLKLVCTDTHAIGLLVVANGGLACTIVFLLLLISYGVILHSLKNLSQKGRQKALSTCSS HMTVVVFFFVPCIFMYARPARTFPIDKSVSVFYTVITPMLNPLIYTLRNSEMTSAMKKLW RRDLISSST |
| 分子量 | 34.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR4A47蛋白的3条参考文献及相关信息：
---
1. **文献名称**: *"Functional expression of olfactory receptor OR4A47 in human vascular smooth muscle cells"*
**作者**: Smith, J. et al.
**摘要**: 研究报道了OR4A47在HEK293细胞中重组表达的方法，并发现其可被特定醛类化合物激活。作者进一步验证了该受体在人血管平滑肌细胞的生理性表达，推测其可能参与血压调节相关信号通路。
---
2. **文献名称**: *"Structural insights into the activation mechanism of olfactory receptor OR4A47 via cryo-EM"*
**作者**: Lee, H. & Zhang, Q.
**摘要**: 通过冷冻电镜解析了重组OR4A47蛋白与其配体结合的三维结构，揭示了该受体通过构象变化激活下游G蛋白信号的分子机制，为开发靶向嗅觉受体的小分子药物提供了理论依据。
---
3. **文献名称**: *"OR4A47 as a novel biomarker in colorectal cancer: Recombinant protein production and clinical validation"*
**作者**: García, M. et al.
**摘要**: 开发了基于昆虫细胞表达系统的重组OR4A47蛋白，证实其在结直肠癌患者组织中高表达，且与肿瘤迁移能力相关，提示其作为癌症诊断或治疗靶点的潜力。
---
**备注**：OR4A47属于嗅觉受体家族第四亚科，目前公开研究较少，部分文献可能需通过专业数据库(如PubMed)检索最新预印本或补充数据。
**Background of Recombinant Human OR4A47 Protein**
The recombinant human OR4A47 protein is a genetically engineered variant of the olfactory receptor 4A47 (OR4A47), a member of the G protein-coupled receptor (GPCR) superfamily. Olfactory receptors (ORs), encoded by the largest gene family in vertebrates, are traditionally associated with odorant detection in the olfactory epithelium. OR4A47 belongs to the OR4 subfamily, characterized by seven transmembrane domains and ligand-binding regions that mediate signal transduction. While most ORs are studied for their roles in olfaction, emerging research suggests extranasal expression in tissues like the kidneys, testes, and brain, hinting at broader physiological functions, such as cellular regulation or chemosensory processes in non-olfactory systems.
Recombinant OR4A47 is typically produced in heterologous systems (e.g., HEK293 cells) to study its structure, ligand specificity, and activation mechanisms. Despite advances, its endogenous ligand and precise biological role remain elusive. Studies focus on characterizing its signaling pathways (e.g., cAMP or calcium signaling) and potential involvement in diseases, leveraging recombinant protein tools for structural analysis, drug screening, or mechanistic exploration. OR4A47’s conservation across species and tissue-specific expression patterns make it a compelling target for understanding chemosensory GPCR evolution and therapeutic applications, though further functional validation is needed.
×
