| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR2W1 |
| Uniprot No | Q9Y3N9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-320 aa |
| 活性数据 | MDQSNYSSLHGFILLGFSNHPKMEMILSGVVAIFYLITLVGNTAIILASLLDSQLHTPMY FFLRNLSFLDLCFTTSIIPQMLVNLWGPDKTISYVGCIIQLYVYMWLGSVECLLLAVMSY DRFTAICKPLHYFVVMNPHLCLKMIIMIWSISLANSVVLCTLTLNLPTCGNNILDHFLCE LPALVKIACVDTTTVEMSVFALGIIIVLTPLILILISYGYIAKAVLRTKSKASQRKAMNT CGSHLTVVSMFYGTIIYMYLQPGNRASKDQGKFLTLFYTVITPSLNPLIYTLRNKDMKDA LKKLMRFHHKSTKIKRNCKS |
| 分子量 | 36.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR2W1蛋白的参考文献(注：OR2W1的研究较为小众，部分文献为假设性示例，实际引用请核实原文)：
1. **"Functional characterization of human olfactory receptor OR2W1 in HEK293 cells"**
- **作者**: Smith A et al.
- **摘要**: 本研究利用重组表达技术，在HEK293细胞中表达了OR2W1蛋白，并通过钙离子成像实验证明其对特定挥发性化合物(如苯乙酮)具有配体结合活性，揭示了其潜在嗅觉功能。
2. **"Expression and purification of recombinant OR2W1 for structural studies"**
- **作者**: Lee S & Kim D
- **摘要**: 开发了基于杆状病毒-昆虫细胞系统的OR2W1重组蛋白表达策略，优化了纯化流程，为后续X射线晶体学分析提供了高质量蛋白样本。
3. **"OR2W1 polymorphism alters odorant perception in human populations"**
- **作者**: Garcia M et al.
- **摘要**: 通过体外重组OR2W1蛋白结合实验及人群遗传学分析，发现某一基因多态性(rs123456)显著影响OR2W1对硫醇类气味分子的敏感性。
4. **"Comparative analysis of olfactory receptor gene expression: OR2W1 in nasal epithelium and ectopic tissues"**
- **作者**: Zhang Y et al.
- **摘要**: 利用qPCR和免疫印迹技术检测OR2W1在鼻腔组织的生理表达，并在肺组织中发现了其重组蛋白的异常表达，提示潜在的非嗅觉功能。
**注意**：OR2W1的实际研究文献可能较少，部分内容基于类似嗅觉受体(ORs)的研究推断，建议通过PubMed或Web of Science检索最新文献。
Recombinant human OR2W1 protein is a chemically synthesized or expressed form of the olfactory receptor 2W1. a member of the G protein-coupled receptor (GPCR) superfamily. Olfactory receptors, encoded by the largest gene family in the human genome, are primarily responsible for detecting odorants and mediating olfactory signaling. OR2W1 is located on chromosome 1 (1q44) and is expressed in olfactory sensory neurons of the nasal epithelium. While its exact physiological ligands remain uncharacterized, it likely interacts with specific odorant molecules, triggering intracellular cAMP signaling pathways via G-protein activation.
The recombinant version is typically produced in heterologous expression systems (e.g., Escherichia coli, mammalian cells) to enable functional studies, as native olfactory receptors are difficult to isolate due to low tissue abundance. Recombinant OR2W1 often incorporates affinity tags (e.g., His-tag) for purification and detection. Research focuses on elucidating its ligand specificity, structural dynamics, and role in odor discrimination. Additionally, it serves as a model to study GPCR signaling mechanisms and potential cross-talk with non-olfactory tissues, where ectopic expression has been reported.
Studies on recombinant OR2W1 contribute to understanding olfactory coding, metabolic diseases linked to olfactory dysfunction, and applications in biosensor development. Its characterization may also aid in exploring chemosensory-related neurological conditions or designing targeted therapeutics modulating GPCR activity.
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