| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR2T27 |
| Uniprot No | Q8NH04 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-317 aa |
| 活性数据 | MEQSNYSVYADFILLGLFSNARFPWLLFALILLVFLTSIASNVVKIILIHIDSRLHTPMY FLLSQLSLRDILYISTIVPKMLVDQVMSQRAISFAGCTAQHFLYLTLAGAEFFLLGLMSY DRYVAICNPLHYPVLMSRKICWLIVAAAWLGGSIDGFLLTPVTMQFPFCASREINHFFCE VPALLKLSCTDTSAYETAMYVCCIMMLLIPFSVISGSYTRILITVYRMSEAEGRGKAVAT CSSHMVVVSLFYGAAMYTYVLPHSYHTPEQDKAVSAFYTILTPMLNPLIYSLRNKDVTGA LQKVVGRCVSSGKVTTF |
| 分子量 | 35.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR2T27蛋白的示例参考文献(部分信息为虚构，供参考)：
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1. **"Structural Insights into the Olfactory Receptor OR2T27 via Recombinant Expression"**
*作者*: Zhang, L., et al. (2018)
*摘要*: 本研究通过重组表达系统在HEK293细胞中成功获得OR2T27蛋白，解析了其三维结构，并发现其与特定挥发性有机化合物的结合位点，揭示了其在嗅觉感知中的潜在作用。
2. **"OR2T7: A Novel Biomarker in Cancer Detection via Recombinant Protein-based Assays"**
*作者*: Patel, R., & Kim, S. (2020)
*摘要*: 利用重组OR2T27蛋白开发了一种高灵敏度检测方法，发现其在肺癌患者体液中的异常表达，或可作为早期诊断标志物。
3. **"Functional Characterization of OR2T27 in Non-Olfactory Tissues Using Recombinant Techniques"**
*作者*: Müller, F., et al. (2022)
*摘要*: 通过重组OR2T27蛋白的体外实验，发现其在肝脏组织中的代谢调控功能，暗示该受体可能参与脂质代谢信号通路。
4. **"Advances in Recombinant OR2T27 Production for High-Throughput Screening"**
*作者*: González, A., et al. (2021)
*摘要*: 优化了重组OR2T27蛋白的昆虫细胞表达系统，提高了蛋白产量和稳定性，为大规模药物筛选提供了技术基础。
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**注意**：以上文献为示例，实际研究中请通过 **PubMed/Google Scholar** 等平台，以“OR2T27”或“olfactory receptor family 2”为关键词检索最新论文。部分嗅觉受体研究可能尚未完全揭示OR2T27的功能，建议结合其家族(如OR2T亚家族)的综述性文献扩展阅读。
OR2T27 is a human olfactory receptor belonging to the class A G protein-coupled receptor (GPCR) family, encoded by the OR2T27 gene located on chromosome 1 (1q44). As a member of the largest GPCR subgroup, it plays a critical role in odorant detection by recognizing specific airborne chemical molecules in nasal olfactory neurons. Though structurally characterized by seven transmembrane domains and ligand-binding pockets, its exact endogenous ligands and activation mechanisms remain poorly characterized compared to well-studied olfactory receptors.
Recombinant OR2T27 protein is typically produced using heterologous expression systems (e.g., HEK293 cells) with epitope tags (such as His-tag) to facilitate purification and detection. This engineered version enables in vitro studies of receptor-ligand interactions, downstream signaling via adenylate cyclase inhibition, and structural analyses through crystallography or cryo-EM. Research applications include deciphering odor coding patterns, screening synthetic odorants, and investigating non-olfactory functions in tissues like testes and kidneys where it's ectopically expressed. Current challenges involve maintaining receptor stability outside native membranes and identifying physiological relevant activators/inhibitors. Its study contributes to understanding chemosensory perception evolution and developing biosensor technologies.
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