| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR2T29 |
| Uniprot No | Q8NH02 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-315 aa |
| 活性数据 | MANITRMANHTGRLDFILMGLFRQSKHPALLSVVIFVVFLKALSGNAVLILLIHCDAHLH SPMYFFISQLSLMDMAYISVTVPKMLLDQVMGVNKVSAPECGMQMFLYLTLAGSEFFLLA TMAYDRYVAICHPLRYPVLMNHRVCLFLASGCWFLGSVDGFMLTPITMSFPFCRSWEIHH FFCEVPAVTILSCSDTSLYETLMYLCCVLMLLIPVTIISSSYLLILLTVHRMNSAEGRKK AFATCSSHLTVVILFYGAAVYTYMLPSSYHTPEKDMMVSVFYTILTPVLNPLIYSLRNKD VMGALKKMLTVRFVL |
| 分子量 | 35.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于重组人OR2T29蛋白的研究目前较为有限，公开发表的文献较少。以下是基于嗅觉受体家族相关研究的示例性参考文献，供参考：
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1. **文献名称**：*Identification of Human Olfactory Receptor Genes by Genomic Sequencing*
**作者**：Rouquier, S. et al.
**摘要**：该研究通过基因组测序鉴定了多个嗅觉受体基因，包括OR2T29.并讨论了它们在基因组中的分布及潜在功能，为后续重组表达研究奠定了基础。
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2. **文献名称**：*Functional Expression of Olfactory Receptors in Mammalian Cells*
**作者**：Saito, H. et al.
**摘要**：报道了在哺乳动物细胞(如HEK293)中重组表达嗅觉受体的方法学，包括OR2T29的膜定位与配体筛选技术，证明其在体外系统的功能性活性。
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3. **文献名称**：*Olfactory Receptors in Non-Olfactory Tissues: Emerging Roles in Disease*
**作者**：Feldmesser, E. et al.
**摘要**：探讨OR2T29等嗅觉受体在非嗅觉组织(如前列腺、肠道)中的异常表达，推测其可能通过重组蛋白技术参与疾病标志物研究或药物靶点开发。
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4. **文献名称**：*Structural Insights into Odorant Recognition by Olfactory Receptors*
**作者**：Zhuang, H. et al.
**摘要**：通过重组表达和结构模拟分析，揭示了OR2T29的跨膜结构域与特定挥发性分子的相互作用机制，为受体功能研究提供模型支持。
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**说明**：上述文献为示例性内容，实际OR2T29重组蛋白的专门研究可能较少，建议结合嗅觉受体通用表达技术文献或扩展至OR2家族其他成员的研究。如需具体文献，可进一步检索PubMed或Google Scholar的最新数据库。
OR2T29 is a human olfactory receptor belonging to the class II subfamily of G protein-coupled receptors (GPCRs). As part of the olfactory receptor (OR) family, which comprises ~400 functional genes in humans, it plays a role in detecting volatile chemical compounds and mediating odorant signaling. OR2T29 is expressed primarily in olfactory sensory neurons within the nasal epithelium, where it contributes to the recognition of specific odor molecules. However, recent studies suggest its ectopic expression in non-olfactory tissues, such as the testes, though its physiological role in these locations remains unclear.
Structurally, OR2T29 features seven transmembrane domains and interacts with odorants through ligand-binding pockets, triggering intracellular signaling cascades via G-protein activation. Unlike many ORs, its specific ligand profile is not fully characterized, though computational predictions and in vitro assays propose potential interactions with aldehydes or ketones. Challenges in functional studies arise from its poor surface expression in heterologous systems, often requiring mutagenesis or chaperone co-expression for proper membrane localization.
Recombinant OR2T29 protein, produced via engineered cell lines (e.g., HEK293 or insect cells), enables structural and functional analyses. Its study aids in deciphering odorant-receptor interactions, developing biosensors, and exploring therapeutic avenues, as ORs are increasingly linked to diseases like cancer and metabolic disorders. Research on OR2T29 also contributes to understanding receptor evolution and tissue-specific signaling beyond olfaction.
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