| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR4C15 |
| Uniprot No | Q8NGM1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-316 aa |
| 活性数据 | MQNQSFVTEFVLLGLSQNPNVQEIVFVVFLFVYIATVGGNMLIVVTILSSPALLVSPMYF FLGFLSFLDACFSSVITPKMIVDSLYVTKTISFEGCMMQLFAEHFFAGVEVIVLTAMAYD RYVAICKPLHYSSIMNRRLCGILMGVAWTGGLLHSMIQILFTFQLPFCGPNVINHFMCDL YPLLELACTDTHIFGLMVVINSGFICIINFSLLLVSYAVILLSLRTHSSEGRWKALSTCG SHIAVVILFFVPCIFVYTRPPSAFSLDKMAAIFYIILNPLLNPLIYTFRNKEVKQAMRRI WNRLMVVSDEKENIKL |
| 分子量 | 35.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR4C15蛋白的3篇假想参考文献，供参考：
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1. **文献名称**: *Heterologous Expression and Functional Characterization of Olfactory Receptor OR4C15 in HEK293 Cells*
**作者**: Smith A, et al.
**摘要**: 该研究成功在HEK293细胞中表达重组OR4C15蛋白，通过钙离子流实验筛选潜在配体，发现其对特定挥发性醛类化合物有选择性响应，表明OR4C15可能在化学传感信号通路中发挥作用。
2. **文献名称**: *OR4C15 Promotes Metastasis in Colorectal Cancer via G-Protein Coupled Signaling*
**作者**: Zhang L, et al.
**摘要**: 研究利用重组OR4C15蛋白发现其在结肠癌细胞中异常高表达，通过激活MAPK/ERK通路增强细胞迁移能力，提示OR4C15或成为癌症治疗的新靶点。
3. **文献名称**: *Structural Insights into OR4C15 Activation Mechanism through Cryo-EM Analysis*
**作者**: Brown K, et al.
**摘要**: 通过冷冻电镜解析重组OR4C15蛋白的三维结构，揭示其与Gαs蛋白偶联的构象变化，为设计靶向嗅觉受体的调节剂提供结构基础。
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**注**：以上为模拟内容，实际文献需通过数据库(如PubMed、Google Scholar)检索确认。若OR4C15相关研究有限，可扩展至嗅觉受体家族(OR4)或重组GPCR表达技术的研究。
OR4C15 (Olfactory Receptor Family 4 Subfamily C Member 15) is a protein encoded by the OR4C15 gene in humans, belonging to the olfactory receptor (OR) superfamily. Olfactory receptors are G protein-coupled receptors (GPCRs) that detect odorant molecules, playing a central role in odor perception. While most ORs are expressed in olfactory sensory neurons, recent studies suggest ectopic expression of OR4C15 in non-olfactory tissues, including the brain, kidney, and reproductive organs, hinting at potential non-sensory roles in cellular signaling or disease pathways. However, its exact physiological function remains poorly characterized.
Recombinant OR4C15 protein is artificially expressed in heterologous systems (e.g., bacterial, insect, or mammalian cells) to enable functional studies. Challenges include its complex transmembrane structure (7-TM domains) and low natural abundance. Researchers often fuse it with solubility tags (e.g., GFP, His-tag) to enhance stability and purification efficiency. Current applications focus on deorphaning OR4C15 (identifying its activating ligands) and exploring its involvement in conditions like cancer (e.g., upregulated in prostate cancer) or metabolic disorders. Structural analysis and ligand-binding assays using recombinant protein aim to elucidate its signaling mechanisms. Despite progress, OR4C15's endogenous ligands and precise biological relevance require further investigation, highlighting its status as an understudied OR with therapeutic or diagnostic potential.
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