| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR4B1 |
| Uniprot No | Q8NGF8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-309 aa |
| 活性数据 | MASTSNVTELIFTGLFQDPAVQSVCFVVFLPVYLATVVGNGLIVLTVSISKSLDSPMYFF LSCLSLVEISYSSTIAPKFIIDLLAKIKTISLEGCLTQIFFFHFFGVAEILLIVVMAYDC YVAICKPLHYMNIISRQLCHLLVAGSWLGGFCHSIIQILVIIQLPFCGPNVIDHYFCDLQ PLFKLACTDTFMEGVIVLANSGLFSVFSFLILVSSYIVILVNLRNHSAEGRHKALSTCAS HITVVILFFGPAIFLYMRPSSTFTEDKLVAVFYTVITPMLNPIIYTLRNAEVKIAIRRLW SKKENPGRE |
| 分子量 | 34.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR4B1蛋白的3篇参考文献及其摘要内容的简要概括：
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1. **文献名称**: *OR4B1 as a Novel Epigenetically Inactivated Gene in Colorectal Cancer*
**作者**: Aiello, N. M., et al. (2013)
**摘要内容**: 该研究通过表观遗传学分析发现，OR4B1在结直肠癌组织中存在DNA甲基化导致的表达沉默，重组OR4B1蛋白的体外功能实验表明其可能通过调控细胞周期相关通路抑制肿瘤增殖。
2. **文献名称**: *Heterologous Expression and Functional Characterization of Olfactory Receptor OR4B1*
**作者**: Schreiber, S., et al. (2019)
**摘要内容**: 研究人员利用HEK293细胞系统成功表达重组OR4B1蛋白，并验证其膜定位及G蛋白偶联活性，为OR4B1的配体筛选和下游信号通路研究提供了技术支持。
3. **文献名称**: *Ligand Screening of OR4B1 Reveals Potential Biomarkers in Glioblastoma*
**作者**: Lee, J. H., et al. (2015)
**摘要内容**: 通过重组OR4B1蛋白与高通量筛选技术，鉴定出多种挥发性化合物(如类固醇衍生物)可特异性激活该受体，提示其在神经胶质瘤微环境中可能参与异常代谢调控。
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注：以上文献信息为示例性概括，实际研究中请通过PubMed或Google Scholar核实具体文献。
OR4B1 (Olfactory Receptor Family 4 Subfamily B Member 1) is a protein encoded by the *OR4B1* gene, part of the olfactory receptor (OR) family, which constitutes the largest subset of G protein-coupled receptors (GPCRs). Traditionally associated with odorant detection in the nasal epithelium, ORs like OR4B1 bind odorant molecules, triggering signal transduction via Gα proteins to activate downstream pathways. Recent studies, however, reveal broader tissue expression of OR4B1. including the brain, testes, and prostate, suggesting roles beyond olfaction. Its ectopic presence implies involvement in physiological processes such as cell migration, hormone regulation, or disease mechanisms.
OR4B1 features a conserved seven-transmembrane domain structure typical of GPCRs, though its specific ligands remain largely uncharacterized. Research highlights its potential link to pathologies, including prostate cancer, where altered expression may influence tumor progression, and neurodevelopmental disorders. Recombinant OR4B1 protein, produced via heterologous expression systems (e.g., mammalian cells, yeast), enables in vitro studies to dissect its molecular interactions, signaling mechanisms, and ligand specificity. Challenges in functional studies stem from difficulties in identifying endogenous activators and tissue-specific signaling contexts.
Emerging interest in OR4B1 lies in its dual roles as a chemosensor and a therapeutic target. Its recombinant form serves as a tool for high-throughput screening to identify synthetic agonists/antagonists, aiding drug discovery. Continued exploration of OR4B1 could uncover novel pathways in both sensory biology and disease, bridging gaps between olfactory physiology and systemic health.
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