| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR52E6 |
| Uniprot No | Q96RD3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-313 aa |
| 活性数据 | MPIANDTQFHTSSFLLLGIPGLEDVHIWIGFPFFSVYLIALLGNAAIFFVIQTEQSLHEP MYYCLAMLDSIDLSLSTATIPKMLGIFWFNIKEISFGGYLSQMFFIHFFTVMESIVLVAM AFDRYIAICKPLWYTMILTSKIISLIAGIAVLRSLYMVIPLVFLLLRLPFCGHRIIPHTY CEHMGIARLACASIKVNIMFGLGSISLLLLDVLLIILSHIRILYAVFCLPSWEARLKALN TCGSHIGVILAFSTPAFFSFFTHCFGHDIPQYIHIFLANLYVVVPPTLNPVIYGVRTKHI RETVLRIFFKTDH |
| 分子量 | 35.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR52E6蛋白的3篇示例参考文献(注：文献为虚构示例，仅作参考模板，实际研究需查阅真实数据库)：
1. **文献名称**：*"Heterologous Expression and Functional Characterization of Human Olfactory Receptor OR52E6 in HEK293 Cells"*
**作者**：Smith A, et al.
**摘要**：该研究成功在HEK293细胞中表达重组OR52E6蛋白，验证其细胞膜定位，并发现其对特定短链脂肪酸的配体结合活性，提示其在代谢调控中的潜在作用。
2. **文献名称**：*"OR52E6 as a Novel Biomarker in Colorectal Cancer: Role of Recombinant Protein in Cell Proliferation Assays"*
**作者**：Zhang Y, et al.
**摘要**：通过重组OR52E6蛋白体外实验，证明其过表达可抑制结直肠癌细胞增殖，可能与Wnt/β-catenin信号通路相互作用相关。
3. **文献名称**：*"Structural Insights into OR52E6 via Recombinant Protein Crystallography"*
**作者**：Lee J, et al.
**摘要**：利用重组OR52E6蛋白解析其三维晶体结构，揭示了该受体独特的跨膜结构域构象，为设计靶向小分子提供依据。
**提示**：实际研究中建议通过PubMed或Google Scholar以“OR52E6 recombinant protein”“OR52E6 expression”等关键词检索最新文献，并关注《Chemical Senses》《BMC Biochemistry》等相关期刊。
**Background of Recombinant Human OR52E6 Protein**
OR52E6. a member of the olfactory receptor (OR) family, belongs to the Class A G protein-coupled receptor (GPCR) superfamily. ORs are typically involved in detecting odorants, but many, including OR52E6. exhibit ectopic expression in non-olfactory tissues, suggesting roles beyond chemosensation. The *OR52E6* gene, located on chromosome 11p15.4. encodes a seven-transmembrane domain receptor with ligand-binding specificity shaped by its unique structural motifs. Though its endogenous ligand remains unidentified, OR52E6 is hypothesized to interact with small hydrophobic molecules, akin to other ORs.
Notably, OR52E6 has been detected in tissues such as the kidney, prostate, and respiratory epithelium, hinting at potential physiological or pathological functions. Studies suggest its involvement in cellular processes like proliferation or inflammation, though mechanistic insights are limited. In cancer contexts, OR52E6 mRNA overexpression has been observed in prostate tumors, sparking interest in its utility as a biomarker or therapeutic target.
Recombinant OR52E6 protein, often produced in heterologous systems (e.g., HEK293 cells), enables functional studies, including ligand screening and signal pathway analysis. Its purification typically incorporates tags (e.g., His-tag) for detection and affinity-based isolation. Current research focuses on deorphanizing OR52E6. elucidating its biological roles, and exploring its therapeutic potential in diseases like cancer or metabolic disorders.
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