| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR52N4 |
| Uniprot No | Q8NGI2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-321 aa |
| 活性数据 | MLTLNKTDLIPASFILNGVPGLEDTQLWISFPFCSMYVVAMVGNCGLLYLIHYEDALHKP MYYFLAMLSFTDLVMCSSTIPKALCIFWFHLKDIGFDECLVQMFFTHTFTGMESGVLMLM ALDRYVAICYPLRYSTILTNPVIAKVGTATFLRGVLLIIPFTFLTKLLPYCRGNILPHTY CDHMSVAKLSCGNVKVNAIYGLMVALLIWGFDILCITNSYTMILRAVVSLSSADARQKAF NTCTAHICAIVFSYTPAFFSFFSHRFGEHIIPPSCHIIVANIYLLLPPTMNPIVYGVKTK QIRDCVIRILSGSKDTKSYSM |
| 分子量 | 36.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是几篇与重组人OR52N4蛋白相关的模拟参考文献(具体文献需通过学术数据库验证)：
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1. **文献名称**：*Functional characterization of recombinant human olfactory receptor OR52N4 expressed in HEK293 cells*
**作者**：Zhang Y, et al. (2018)
**摘要**：研究成功在HEK293细胞中表达了重组OR52N4蛋白，通过钙离子成像实验证实其对特定短链脂肪酸的配体响应，提示其可能参与非嗅觉组织的化学感知功能。
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2. **文献名称**：*OR52N4 and prostate cancer: Expression analysis and potential therapeutic target*
**作者**：Smith JL, et al. (2020)
**摘要**：首次在前列腺癌细胞中检测到OR52N4高表达，并发现其激活可抑制癌细胞增殖，可能与雄激素受体信号通路存在交叉调控。
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3. **文献名称**：*Structural insights into OR52N4 activation mechanism via cryo-EM*
**作者**：Lee S, et al. (2021)
**摘要**：利用冷冻电镜解析重组OR52N4蛋白的三维结构，揭示了其配体结合口袋的构象变化及下游G蛋白偶联的分子机制。
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4. **文献名称**：*Comparative study of OR52N4 expression in CHO vs. insect cell systems*
**作者**：Brown K, et al. (2019)
**摘要**：比较两种重组表达系统对OR52N4蛋白活性的影响，发现CHO系统更利于维持其天然构象和配体响应功能。
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**注意事项**：
- 上述文献为基于研究领域常见主题的模拟内容，实际文献需通过 **PubMed/Google Scholar** 检索验证。
- 推荐关键词：`OR52N4 recombinant expression`, `OR52N4 ligand`, `OR52N4 cancer`。
- 若文献较少，可扩大至嗅觉受体家族(OR52亚家族)的共性研究。
**Background of Recombinant Human OR52N4 Protein**
The recombinant human OR52N4 protein is a functionally expressed olfactory receptor (OR) belonging to the OR52 subfamily within the class A G protein-coupled receptor (GPCR) superfamily. Olfactory receptors are pivotal in detecting odorant molecules and mediating signal transduction via interactions with G proteins, typically initiating cAMP or inositol phosphate signaling pathways. OR52N4. encoded by the *OR52N4* gene located on chromosome 11. is characterized by its seven-transmembrane domain structure, a hallmark of GPCRs. While the exact physiological ligand for OR52N4 remains under investigation, it is hypothesized to respond to specific volatile compounds, contributing to the diverse human olfactory perception spectrum.
Recombinant production of OR52N4 enables detailed structural and functional studies, overcoming challenges such as low natural expression levels in native tissues. This protein is commonly expressed in heterologous systems (e.g., HEK293 cells) using viral vectors or synthetic constructs, often tagged with fluorescent proteins or epitopes for purification and tracking. Its recombinant form aids in deorphanization efforts (identifying activating ligands) and exploring roles beyond olfaction, including potential involvement in non-olfactory tissues, where ORs may regulate processes like cell migration or metabolism.
Research on OR52N4 also supports drug discovery, as olfactory receptors are emerging targets for conditions linked to chemosensory dysfunction or metabolic disorders. However, challenges persist in stabilizing membrane-bound ORs *in vitro* and deciphering ligand-receptor specificity. Advances in cryo-EM and computational modeling are accelerating mechanistic insights, positioning OR52N4 as a model for understanding GPCR diversity and therapeutic potential.
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