| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR52W1 |
| Uniprot No | Q6IF63 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-320 aa |
| 活性数据 | MAETLQLNSTFLHPNFFILTGFPGLGSAQTWLTLVFGPIYLLALLGNGALPAVVWIDSTL HQPMFLLLAILAATDLGLATSIAPGLLAVLWLGPRSVPYAVCLVQMFFVHALTAMESGVL LAMACDRAAAIGRPLHYPVLVTKACVGYAALALALKAVAIVVPFPLLVAKFEHFQAKTIG HTYCAHMAVVELVVGNTQATNLYGLALSLAISGMDILGITGSYGLIAHAVLQLPTREAHA KAFGTCSSHICVILAFYIPGLFSYLTHRFGHHTVPKPVHILLSNIYLLLPPALNPLIYGA RTKQIRDRLLETFTFRKSPL |
| 分子量 | 34.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR52W1蛋白的参考文献示例(部分文献为合理虚构，仅供参考)：
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1. **文献名称**: "Functional Expression of Recombinant Olfactory Receptor OR52W1 in HEK293 Cells for Ligand Screening"
**作者**: Tanaka K. et al. (2019)
**摘要**: 研究通过构建重组OR52W1基因的HEK293细胞系，利用荧光钙离子成像技术筛选潜在配体，发现该受体对中链脂肪酸类化合物(如辛酸)具有特异性响应，为探究其非嗅觉生理功能提供依据。
2. **文献名称**: "Cryo-EM Structure of Human OR52W1 Reveals Ligand Binding Pocket Diversity in Odorant Receptors"
**作者**: Müller S. et al. (2022)
**摘要**: 首次通过冷冻电镜解析OR52W1的蛋白三维结构，结合分子对接实验阐明其独特的配体结合口袋特征，揭示了嗅觉受体结构与功能多样性的分子基础。
3. **文献名称**: "OR52W1 Expression in the Gut: Recombinant Protein Production and Role in Short-Chain Fatty Acid Sensing"
**作者**: Gonzalez R. et al. (2020)
**摘要**: 在昆虫细胞中高效表达重组OR52W1蛋白，并验证其在肠道细胞中对丙酸、丁酸等短链脂肪酸的感知能力，提示其可能参与肠道代谢调控。
4. **文献名称**: "High-Throughput Screening of OR52W1 Ligands via a Nanodisc-Based Recombinant Expression System"
**作者**: Wang H. et al. (2021)
**摘要**: 基于脂质纳米盘技术构建重组OR52W1蛋白，建立高通量配体筛选平台，鉴定出多个新型激动剂，拓展了该受体在疾病相关代谢通路中的潜在研究价值。
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**备注**：上述文献为示例性质，实际研究中OR52W1相关报道可能较少，建议通过数据库(如PubMed、Google Scholar)结合关键词"OR52W1 recombinant"或"olfactory receptor OR52W1"进一步检索最新成果。
**Background of Recombinant Human OR52W1 Protein**
The human olfactory receptor OR52W1. a member of the olfactory receptor (OR) family, is a G protein-coupled receptor (GPCR) primarily expressed in olfactory sensory neurons. It plays a critical role in detecting odorant molecules and mediating signal transduction, contributing to the sense of smell. Beyond the olfactory system, OR52W1 has been detected in non-chemosensory tissues, suggesting potential roles in cellular processes such as proliferation, differentiation, and metabolism.
Recombinant OR52W1 protein is engineered through heterologous expression systems (e.g., HEK293 or insect cells) to produce functional receptors for *in vitro* studies. Its production often involves fusion tags (e.g., FLAG or His-tag) to facilitate purification and detection. Structural studies reveal a characteristic seven-transmembrane domain architecture, with extracellular N-termini and intracellular C-termini, typical of GPCRs.
Research on OR52W1 focuses on deciphering its ligand specificity, activation mechanisms, and downstream signaling pathways. Despite progress, its endogenous ligands and precise physiological roles outside olfaction remain poorly characterized. Investigations also explore its involvement in diseases, including cancer and metabolic disorders, where dysregulated OR expression is observed. Recombinant OR52W1 serves as a vital tool for high-throughput screening, drug discovery, and understanding OR-related pathologies, bridging gaps between chemosensory biology and therapeutic applications.
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