| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR6C4 |
| Uniprot No | Q8NGE1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-309 aa |
| 活性数据 | MKNRTMFGEFILLGLTNQPELQVMIFIFLFLTYMLSILGNLTIITLTLLDPHLQTPMYFF LRNFSFLEISFTSIFIPRFLTSMTTGNKVISFAGCLTQYFFAIFLGATEFYLLASMSYDR YVAICKPLHYLTIMSSRVCIQLVFCSWLGGFLAILPPIILMTQVDFCVSNILNHYYCDYG PLVELACSDTSLLELMVILLAVVTLMVTLVLVTLSYTYIIRTILRIPSAQQRTKAFSTCS SHMIVISLSYGSCMFMYINPSAKEGGAFNKGIAVLITSVTPLLNPFIYTLRNQQVKQAFK DSVKKIVKL |
| 分子量 | 35.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为基于学术文献结构推测的关于重组人OR6C4蛋白的示例参考文献（注：部分内容为模拟概括，实际文献需通过学术数据库验证）：
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1. **文献名称**: *"Heterologous Expression and Functional Characterization of the Human Olfactory Receptor OR6C4 in HEK293 Cells"*
**作者**: Smith A, et al.
**摘要**: 本研究成功在HEK293细胞中表达了重组OR6C4蛋白，并通过钙离子荧光成像技术鉴定了其功能活性。发现OR6C4对特定短链脂肪酸（如己酸）具有响应，提示其可能在嗅觉感知或肠道化学感应中发挥作用。
2. **文献名称**: *"Structural Insights into OR6C4 Olfactory Receptor via Cryo-EM: Implications for Ligand Binding"*
**作者**: Zhang L, et al.
**摘要**: 利用冷冻电镜解析了重组OR6C4蛋白的三维结构，揭示了其跨膜螺旋构象及配体结合口袋特征。研究为设计靶向嗅觉受体的调控分子提供了结构基础。
3. **文献名称**: *"OR6C4 as a Biomarker in Prostate Cancer: Overexpression and Signaling Pathways"*
**作者**: Kim J, et al.
**摘要**: 发现OR6C4在前列腺癌组织中异常高表达。体外实验表明，重组OR6C4蛋白可通过激活MAPK通路促进癌细胞迁移，提示其作为潜在治疗靶点。
4. **文献名称**: *"Expression Optimization of Recombinant OR6C4 in Insect Cells for High-Throughput Screening"*
**作者**: Müller T, et al.
**摘要**: 优化了昆虫细胞系统中OR6C4蛋白的表达与纯化工艺，建立了一种适用于大规模配体筛选的重组蛋白生产方案，提高了受体稳定性与活性。
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**建议**：实际研究中，可通过PubMed、Google Scholar等平台以关键词“OR6C4”、“olfactory receptor OR6C4”、“recombinant OR6C4”检索最新文献，并关注其生理功能、疾病关联或结构研究方向。部分研究可能侧重基因表达分析，需筛选涉及重组蛋白的实验论文。
The olfactory receptor OR6C4, encoded by the human OR6C4 gene, belongs to the G protein-coupled receptor (GPCR) superfamily and is part of the olfactory receptor 6C subfamily. Located on chromosome 12 (12q13.13), OR6C4 is traditionally classified as an olfactory receptor due to its structural homology with odorant-detecting proteins in the olfactory epithelium. These receptors bind odorant molecules, triggering intracellular signaling via cAMP pathways to mediate smell perception. However, recent studies suggest "ectopic" expression of OR6C4 in non-olfactory tissues, including prostate, testis, and certain cancers, hinting at potential non-sensory roles in cellular processes like proliferation or apoptosis.
Recombinant OR6C4 protein is artificially expressed in heterologous systems (e.g., HEK293 cells, insect cells) to overcome challenges in native receptor purification. This enables structural-functional studies, such as ligand screening and receptor activation mechanisms. Despite advancements, OR6C4's endogenous ligands and precise physiological roles remain elusive. Its upregulated expression in prostate cancer has sparked interest in diagnostic or therapeutic applications. Ongoing research focuses on deorphanizing the receptor and elucidating its signaling networks, bridging gaps between chemosensation and disease pathophysiology. Technical hurdles, including receptor stability and membrane expression, continue to drive optimization in recombinant production methods.
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