| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR7D2 |
| Uniprot No | Q96RA2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-312 aa |
| 活性数据 | MEAGNQTGFLEFILLGLSEDPELQPFIFGLFLSMYLVTVLGNLLIILAISSDSHLHTPMY FFLSNLSWVDICFSTCIVPKMLVNIQTENKAISYMDCLTQVYFSMFFPILDTLLLTVMAY DRFVAVCHPLHYMIIMNPHLCGLLVFVTWLIGVMTSLLHISLMMHLIFCKDFEIPHFFCE LTYILQLACSDTFLNSTLIYFMTGVLGVFPLLGIIFSYSRIASSIRKMSSSGGKQKALST CGSHLSVVSLFYGTGIGVHFTSAVTHSSQKISVASVMYTVVTPMLNPFIYSLRNKDVKGA LGSLLSRAASCL |
| 分子量 | 34.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR7D2蛋白的3篇参考文献的示例（注：文献信息为假设性概括，具体内容需结合实际数据库验证）：
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1. **文献名称**: "Comprehensive Functional Characterization of Human Olfactory Receptor OR7D2 Reveals Specific Ligand Selectivity"
**作者**: Müller, C.E. et al.
**摘要**: 该研究通过异源表达系统成功制备了重组OR7D2蛋白，并鉴定其对特定类固醇气味分子（如雄烯酮）的高亲和力，揭示了其在人体嗅觉感知中的潜在作用。
2. **文献名称**: "Heterologous Expression and Purification of the Human Olfactory Receptor OR7D2 in HEK293 Cells"
**作者**: Zhang, Y. et al.
**摘要**: 文章优化了OR7D2在HEK293细胞中的重组表达与纯化流程，通过荧光标记和表面等离子共振技术（SPR）分析其结构与膜定位特性，为后续功能研究提供方法基础。
3. **文献名称**: "OR7D2 Activation Modulates Sperm Chemotaxis: Implications for Human Reproductive Biology"
**作者**: Fukuda, N. et al.
**摘要**: 该研究发现OR7D2在睾丸组织中异常表达，重组蛋白实验表明其参与调控精子细胞的趋化运动，提示其可能在生殖生理中具有嗅觉感知外的非经典功能。
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**注意**：以上文献为示例，实际引用时建议通过PubMed、Web of Science等平台检索最新研究，并使用真实发表数据。OR7D2研究相对较少，可扩展至嗅觉受体家族共性技术或相关GPCR重组表达方法的文献。
**Background of Recombinant Human OR7D2 Protein**
OR7D2, a member of the olfactory receptor (OR) family, belongs to the G protein-coupled receptor (GPCR) superfamily. It is encoded by the *OR7D2* gene, located on human chromosome 19. Olfactory receptors detect odorant molecules, mediating sensory signaling in the nasal epithelium. Although OR7D2’s natural ligand remains unidentified, it is hypothesized to recognize specific volatile compounds, contributing to odor perception.
Recombinant OR7D2 protein is artificially produced using heterologous expression systems (e.g., HEK293 or insect cells) to study its structure, ligand-binding properties, and downstream signaling. Its production often involves tagging (e.g., His-tag) for purification via affinity chromatography. As a membrane protein, OR7D2 presents challenges in solubilization and stabilization, requiring detergents or lipid-based systems for functional studies.
Research on OR7D2 aims to elucidate its role in olfaction, potential non-olfactory functions (e.g., tissue-specific expression in other organs), and interactions with signaling pathways. Its recombinant form serves as a tool for high-throughput screening of odorants or therapeutic candidates targeting GPCRs. However, functional characterization remains limited due to incomplete ligand identification and low natural expression levels. Advances in structural biology (e.g., cryo-EM) may enhance understanding of its activation mechanisms and therapeutic relevance.
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