| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR6N2 |
| Uniprot No | Q8NGY6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-317 aa |
| 活性数据 | MDQYNHSSLAEFVFLGFASVGYVRGWLFVLLLLAYLFTICGNMLIFSVIRLDAALHTPMY HFVSVLSFLELWYTATTIPKMLSNILSEKKTISFAGCLLQTYFFHSLGASECYLLTAMAY DRYLAICRPLHYPIIMTTTLCAKMAAACWTCGFLCPISEVILASQLPFCAYNEIQHIFCD FPPLLSLACKDTSANILVDFAINAFIILITFFFIMISYARIIGAVLKIKTASGRKKAFST CASHLAVVLIFFGSIIFMYVRLKKSYSLTLDRTLAIVYSVLTPMVNPIIYSLRNKEIIKA IKRTIFQKGDKASLAHL |
| 分子量 | 35.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于典型嗅觉受体重组蛋白研究模式的模拟参考文献（注：OR6N2公开文献较少，以下为近似研究示例框架）：
1. **"Functional expression of human olfactory receptor OR6N2 in HEK293 cells"**
- 作者: Smith J, et al.
- 摘要: 开发了重组OR6N2蛋白在HEK293细胞中的真核表达系统，利用荧光钙成像技术检测到其对特定脂肪醛类分子的响应，证明其在体外具有气味识别功能。
2. **"Structural analysis of OR6N2 olfactory receptor using cryo-EM"**
- 作者: Zhang L, et al.
- 摘要: 通过冷冻电镜解析重组OR6N2蛋白与配体复合物的三维结构，揭示其跨膜结构域内的关键氨基酸残基在气味结合中的构象变化机制。
3. **"OR6N2 variants alter fatty acid perception in humans"**
- 作者: Garcia M, et al.
- 摘要: 研究OR6N2基因多态性对气味感知的影响，通过重组蛋白实验证明第90位谷氨酰胺突变为精氨酸会显著改变其对C9-C12醛类化合物的结合亲和力。
4. **"High-throughput screening of OR6N2 ligands via nanodisc-embedded receptor system"**
- 作者: Tanaka K, et al.
- 摘要: 建立基于纳米脂质盘技术的重组OR6N2高通量筛选平台，筛选出包括壬醛和癸醛在内的潜在生理配体，并发现其在嗅觉神经元中的特异性激活现象。
注：目前公开数据库中关于OR6N2的研究数据较为有限，建议扩展检索至嗅觉受体家族整体研究或采用"OR6N2 AND (expression/ligand/structure)"等组合关键词。实际文献请通过PubMed/Google Scholar验证。
OR6N2 is a human olfactory receptor belonging to the G protein-coupled receptor (GPCR) superfamily, specifically classified under the olfactory receptor (OR) subfamily 6. Encoded by the OR6N2 gene located on chromosome 1p36.33, it is expressed primarily in olfactory sensory neurons within the nasal epithelium. Like other olfactory receptors, OR6N2 detects odorant molecules, translating chemical signals into neural activity through cAMP-mediated signaling pathways. Its exact ligand specificity remains unclear, but structural studies suggest it binds to volatile organic compounds, potentially contributing to the perception of specific scents.
Recombinant OR6N2 protein is produced in heterologous expression systems (e.g., HEK293 or insect cells) for functional studies, often tagged with fluorescent or affinity markers for purification and tracking. Research focuses on its role in odor discrimination, receptor-ligand interactions, and potential non-olfactory functions, as some ORs are ectopically expressed in tissues like the brain, heart, or testes. Genetic variations in OR6N2 have been weakly associated with odor sensitivity differences, though its clinical significance is unexplored.
Despite advances, OR6N2 remains undercharacterized compared to other ORs. Challenges include low natural abundance, difficulty in heterologous expression, and unresolved signaling mechanisms. Current applications involve deciphering the olfactory code, designing biosensors, or exploring therapeutic targets linked to chemosensory disorders. Its study exemplifies broader efforts to map the human olfactory repertoire and understand how GPCR diversity underpins sensory perception.
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