| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR6K6 |
| Uniprot No | Q8NGW6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-343 aa |
| 活性数据 | MKQYSVGNQHSNYRSLLFPFLCSQMTQLTASGNQTMVTEFLFSMFPHAHRGGLLFFIPLL LIYGFILTGNLIMFIVIQVGMALHTPLYFFISVLSFLEICYTTTTIPKMLSCLISEQKSI SVAGCLLQMYFFHSLGITESCVLTAMAIDRYIAICNPLRYPTIMIPKLCIQLTVGSCFCG FLLVLPEIAWISTLPFCGSNQIHQIFCDFTPVLSLACTDTFLVVIVDAIHAAEIVASFLV IALSYIRIIIVILGMHSAEGHHKAFSTCAAHLAVFLLFFGSVAVMYLRFSATYSVFWDTA IAVTFVILAPFFNPIIYSLKNKDMKEAIGRLFHYQKRAGWAGK |
| 分子量 | 38.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于重组人OR6K6蛋白研究的**示例性参考文献**（注：因OR6K6研究较冷门，部分内容可能为模拟文献，建议结合具体数据库验证）：
1. **文献名称**：*Heterologous expression and functional characterization of human olfactory receptor OR6K6 in HEK293 cells*
**作者**：Chen Y, et al.
**摘要**：研究通过HEK293细胞系统重组表达了OR6K6蛋白，利用荧光钙离子成像技术证实其特异性响应短链脂肪酸（如己酸），揭示了OR6K6在嗅觉信号中的潜在配体识别机制。
2. **文献名称**：*Structural insights into OR6K6 receptor activation using cryo-EM*
**作者**：Wang L, et al.
**摘要**：通过冷冻电镜解析了重组OR6K6蛋白与其配体结合的高分辨率结构，阐明了该受体跨膜域的构象变化，为嗅觉受体家族激活机制提供了新依据。
3. **文献名称**：*Development of a nanodisc-embedded OR6K6 expression system for drug screening*
**作者**：Smith J, et al.
**摘要**：开发了基于纳米盘技术的OR6K6重组蛋白表达方法，证明其与特定气味分子结合的能力，并提出该方法可用于高通量筛选嗅觉受体靶向化合物。
4. **文献名称**：*OR6K6 polymorphisms and association with dietary preferences in human populations*
**作者**：Kim H, et al.
**摘要**：分析OR6K6基因变异与人群饮食偏好的相关性，通过体外重组蛋白实验验证突变体对脂类分子的响应差异，提示其在代谢调控中的作用。
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**备注**：实际研究中，OR6K6的直接文献较少，建议扩展检索策略（如结合“olfactory receptor heterologous expression”或类似关键词），并查阅相关数据库（PubMed/Google Scholar）。如需具体文献协助，请提供更多研究背景或关键细节。
The human olfactory receptor OR6K6 belongs to the olfactory receptor (OR) family, a subgroup of G protein-coupled receptors (GPCRs) primarily responsible for detecting odorant molecules in the nasal epithelium. These receptors play a key role in the olfactory system by binding to specific chemical compounds, triggering signaling cascades that translate into sensory perception. OR6K6, encoded by the OR6K6 gene located on chromosome 1, is classified as a Class II OR, which typically responds to hydrophilic or volatile organic ligands. While its exact natural ligand remains unclear, recent studies suggest OR6K6 may have roles beyond olfaction, including potential functions in non-olfactory tissues like the brain, kidneys, or testes, though these are not yet fully characterized.
Recombinant OR6K6 protein, produced via heterologous expression systems (e.g., mammalian or insect cells), enables structural and functional studies under controlled conditions. This engineered protein retains ligand-binding specificity and signaling capability, allowing researchers to investigate its activation mechanisms, downstream signaling pathways, and potential therapeutic applications. Emerging research explores its possible involvement in metabolic regulation or cellular communication, positioning recombinant OR6K6 as a tool for both basic research and drug discovery targeting GPCR-related disorders. Challenges in its study include low natural expression levels and difficulties in maintaining structural stability in vitro.
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