| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR6N1 |
| Uniprot No | Q8NGY5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-312 aa |
| 活性数据 | MDTGNWSQVAEFIILGFPHLQGVQIYLFLLLLLIYLMTVLGNLLIFLVVCLDSRLHTPMY HFVSILSFSELGYTAATIPKMLANLLSEKKTISFSGCLLQIYFFHSLGATECYLLTAMAY DRYLAICRPLHYPTLMTPTLCAEIAIGCWLGGLAGPVVEISLISRLPFCGPNRIQHVFCD FPPVLSLACTDTSINVLVDFVINSCKILATFLLILCSYVQIICTVLRIPSAAGKRKAIST CASHFTVVLIFYGSILSMYVQLKKSYSLDYDQALAVVYSVLTPFLNPFIYSLRNKEIKEA VRRQLKRIGILA |
| 分子量 | 34.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR6N1蛋白的3-4篇文献摘要示例（注：文献为虚构示例，实际需查阅数据库补充）：
1. **"Functional expression of recombinant human olfactory receptor OR6N1 in HEK293 cells and its ligand screening"**
*作者：Zhang L. et al. (2020)*
**摘要**：该研究在HEK293细胞中成功重组表达了OR6N1蛋白，并通过钙流实验鉴定其对短链脂肪酸（如己酸）的特异性响应，揭示了其在嗅觉感知中的潜在作用。
2. **"Structural characterization of OR6N1 using cryo-EM reveals odorant-binding mechanisms"**
*作者：Kim S. et al. (2019)*
**摘要**：利用冷冻电镜解析了重组OR6N1蛋白的三维结构，揭示了其与气味分子结合的关键氨基酸位点及跨膜结构域的构象变化机制。
3. **"OR6N1 receptor expression in non-olfactory tissues and its role in metabolic regulation"**
*作者：Garcia R. et al. (2018)*
**摘要**：通过重组OR6N1蛋白体外实验，发现其在脂肪细胞中异常表达，可能参与调节脂质代谢通路，暗示嗅觉受体在嗅觉外系统的功能。
4. **"High-throughput screening of OR6N1 modulators via a recombinant luciferase reporter system"**
*作者：Wang Y. et al. (2021)*
**摘要**：构建基于重组OR6N1的荧光素酶报告系统，筛选出多个小分子激动剂/拮抗剂，为嗅觉受体靶向药物开发提供新工具。
（注：以上文献为示例，实际文献需通过PubMed、Google Scholar等数据库检索确认。）
The OR6N1 protein, part of the olfactory receptor (OR) family, is a G protein-coupled receptor (GPCR) primarily involved in odorant detection. These receptors are located in olfactory sensory neurons, where they recognize odor molecules, initiating signal transduction pathways that translate chemical signals into neural activity. OR6N1 is encoded by the OR6N1 gene on human chromosome 1 and belongs to the largest mammalian gene family, with over 400 functional ORs in humans. Despite its classification as an olfactory receptor, some studies suggest OR6N1 may have non-olfactory roles, including potential expression in sperm cells, hinting at involvement in chemotaxis or reproductive processes.
Recombinant OR6N1 protein is synthesized using heterologous expression systems (e.g., HEK293 cells, insect cell lines) to overcome challenges in native protein isolation due to low natural abundance and membrane-associated complexity. Its production enables structural and functional studies, such as ligand screening, receptor activation mechanisms, and interaction analyses with downstream signaling components. However, OR expression remains technically demanding due to poor solubility and the need for lipid membrane mimics in experimental setups. Emerging research explores OR6N1's potential in biosensor development or as a therapeutic target, leveraging its high specificity for odorant molecules. Characterization of this receptor contributes to understanding olfactory coding and GPCR evolution while offering tools for synthetic biology applications.
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