| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR8B12 |
| Uniprot No | Q8NGG6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-310 aa |
| 活性数据 | MAAKNSSVTEFILEGLTHQPGLRIPLFFLFLGFYTVTVVGNLGLITLIGLNSHLHTPMYF FLFNLSLIDFCFSTTITPKMLMSFVSRKNIISFTGCMTQLFFFCFFVVSESFILSAMAYD RYVAICNPLLYTVTMSCQVCLLLLLGAYGMGFAGAMAHTGSIMNLTFCADNLVNHFMCDI LPLLELSCNSSYMNELVVFIVVAVDVGMPIVTVFISYALILSSILHNSSTEGRSKAFSTC SSHIIVVSLFFGSGAFMYLKPLSILPLEQGKVSSLFYTIIVPVLNPLIYSLRNKDVKVAL RRTLGRKIFS |
| 分子量 | 34.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR8B12蛋白的假设性参考文献示例（基于常见研究方向模拟，非真实文献）：
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1. **标题**：*Functional Characterization of Recombinant Human OR8B12 in Olfactory Signal Transduction*
**作者**：Chen L, et al.
**摘要**：研究利用HEK293细胞成功表达重组人OR8B12蛋白，并通过钙离子成像实验证明其对特定挥发性化合物（如类固醇衍生物）的配体结合活性，揭示了其在嗅觉信号传递中的作用机制。
2. **标题**：*Structural Insights into OR8B12: Cryo-EM Analysis of a Recombinant Olfactory Receptor*
**作者**：Smith J, Wang Y.
**摘要**：通过冷冻电镜技术解析了重组人OR8B12蛋白的三维结构，发现其跨膜域构象变化与配体结合特异性相关，为嗅觉受体家族的结构-功能关系提供了新证据。
3. **标题**：*Recombinant OR8B12 as a Biomarker for Neurodegenerative Disorders*
**作者**：Kim S, et al.
**摘要**：发现阿尔茨海默病患者嗅觉上皮中OR8B12表达显著降低，通过重组蛋白模型证实其与β-淀粉样蛋白的相互作用，提示其作为早期神经退行性疾病标志物的潜力。
4. **标题**：*High-Yield Production of Recombinant OR8B12 in Insect Cells and Its Application in High-Throughput Screening*
**作者**：Rodriguez M, et al.
**摘要**：开发了一种基于杆状病毒表达系统的重组OR8B12大规模生产方法，并应用于高通量药物筛选平台，成功鉴定出新型嗅觉调节分子。
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**说明**：以上文献为示例性模拟，实际研究中OR8B12相关文献较少，建议通过PubMed或Google Scholar以“OR8B12 recombinant protein”或“OR8B12 olfactory receptor”为关键词检索最新成果。
OR8B12 is a member of the olfactory receptor (OR) family, a class of G protein-coupled receptors (GPCRs) that play a central role in detecting odorants and mediating olfactory signaling. Specifically, OR8B12 is encoded by the OR8B12 gene located on human chromosome 11. While most olfactory receptors are predominantly expressed in the nasal olfactory epithelium, some, including OR8B12, show low-level expression in non-olfactory tissues, suggesting potential roles beyond smell perception, though its exact physiological functions remain underexplored. Structurally, OR8B12 features seven transmembrane domains typical of GPCRs, with extracellular and intracellular loops facilitating ligand binding and signal transduction.
Recombinant OR8B12 protein refers to the genetically engineered version produced in heterologous systems (e.g., bacterial, mammalian, or insect cells) for functional studies. Its recombinant expression enables investigations into ligand specificity, activation mechanisms, and downstream signaling pathways. Challenges in producing functional OR8B12 include proper membrane localization and post-translational modifications, often addressed using codon-optimized constructs or specialized expression vectors. Current research focuses on deorphaning OR8B12 by identifying its activating odorants or endogenous ligands, which could clarify its role in chemosensation or non-canonical processes like tissue homeostasis. Additionally, recombinant OR8B12 serves as a tool for studying OR-related diseases or developing biosensors. Its characterization contributes to broader insights into GPCR biology and sensory signal processing.
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