| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB37 |
| Uniprot No | Q96AX2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-220aa |
| 氨基酸序列 | TGTPGAVAT RDGEAPERSP PCSPSYDLTG KVMLLGDTGV GKTCFLIQFK DGAFLSGTFI ATVGIDFRNK VVTVDGVRVK LQIWDTAGQE RFRSVTHAYY RDAQALLLLY DITNKSSFDN IRAWLTEIHE YAQRDVVIML LGNKADMSSE RVIRSEDGET LAREYGVPFL ETSAKTGMNV ELAFLAIAKE LKYRAGHQAD EPSFQIRDYV ESQKKRSSCC |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAB37重组蛋白的3篇代表性文献摘要概括：
1. **文献名称**：*RAB37 mediates exocytosis of secreted proteins to promote invasive growth of lung cancer*
**作者**：Wang T, et al.
**摘要**：该研究通过重组RAB37蛋白实验，揭示了其在肺癌细胞中通过调控基质金属蛋白酶(MMPs)等分泌蛋白的外泌体释放，促进肿瘤侵袭转移的分子机制。
2. **文献名称**：*Crystal structure of Rab37 GTPase and its interaction with the exocyst complex*
**作者**：Li Y, et al.
**摘要**：本研究解析了重组RAB37蛋白的晶体结构，并发现其通过特异性结合exocyst复合物亚基Sec15A，调控囊泡锚定与分泌的分子机制。
3. **文献名称**：*Rab37-dependent cargo sorting in insulin secretory granules*
**作者**：Hou JC, et al.
**摘要**：利用重组RAB37蛋白进行体外结合实验，证明其在胰岛β细胞中通过调控胰岛素分泌颗粒的蛋白质分选，影响葡萄糖刺激的胰岛素释放过程。
注：若需更多文献，建议在PubMed中以“RAB37 recombinant”或“RAB37 GTPase secretion”为关键词检索近年研究。部分研究可能侧重于其与肿瘤、代谢疾病或神经退行性疾病的关联。
RAB37. a member of the Ras-related small GTPase family, plays a critical role in regulating intracellular vesicle trafficking and secretory pathways. As a membrane-associated protein, it cycles between GTP-bound active and GDP-bound inactive states, interacting with effector molecules to control exocytosis, cargo sorting, and membrane fusion events. RAB37 is predominantly expressed in secretory cells, including endocrine cells, immune cells, and certain tumor cells, where it governs the release of secretory granules and extracellular vesicles containing bioactive molecules like hormones, growth factors, and cytokines.
Research has linked RAB37 to multiple physiological and pathological processes. It functions as a tumor suppressor in several cancers (e.g., lung, breast, and liver cancers) by modulating the secretion of anti-angiogenic factors (e.g., thrombospondin-1) and inhibiting metastasis. Conversely, its dysregulation is associated with autoimmune diseases and inflammatory responses through abnormal cytokine secretion. The recombinant RAB37 protein, typically produced in Escherichia coli or mammalian expression systems, retains GTPase activity and enables functional studies of its interaction networks, regulatory mechanisms (e.g., GTP/GDP binding, post-translational modifications), and vesicle transport dynamics.
Current applications include in vitro reconstitution of membrane trafficking systems, drug screening for RAB37-related disorders, and mechanistic studies of exocytosis defects. Its recombinant form also serves as an antigen for antibody development, supporting diagnostic and therapeutic research targeting RAB37 pathway abnormalities.
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