Recombinant Human ARHGEF7 protein

中文名： Rho鸟嘌呤核苷酸交换因子7(ARHGEF7)重组蛋白
别名： ARHGEF7；COOL1；KIAA0142；P85SPR；Rho guanine nucleotide exchange factor 7

货号: PA1000-7864

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	ARHGEF7
Uniprot No	Q14155
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	179-428aa
氨基酸序列	MTDNSNNQLVVRAKFNFQQTNEDELSFSKGDVIHVTRVEEGGWWEGTLNGRTGWFPSNYVREVKASEKPVSPKSGTLKSPPKGFDTTAINKSYYNVVLQNILETENEYSKELQTVLSTYLRPLQTSEKLSSANISYLMGNLEEICSFQQMLVQSLEECTKLPEAQQRVGGCFLNLMPQMKTLYLTYCANHPSAVNVLTEHSEELGEFMETKGASSPGILVLTTGLSKPFMRLDKYPTLLKELERHMEDYH
预测分子量	55.3kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于ARHGEF7重组蛋白的3篇代表性文献(虚构示例，仅作格式参考)：

1. **"ARHGEF7重组蛋白介导Cdc42激活的分子机制研究"**

- 作者：Manser E., Leung T.

- 摘要：研究通过大肠杆菌系统表达并纯化ARHGEF7重组蛋白，发现其通过N端结构域特异性激活Cdc42 GTP酶，调控细胞骨架重组和迁移。

2. **"ARHGEF7磷酸化修饰对其重组蛋白功能的影响"**

- 作者：Rosenberger G., Wittmann T.

- 摘要：利用昆虫细胞表达系统获得高纯度ARHGEF7重组蛋白，证明其Tyr-108位点的磷酸化可增强与PAK激酶的结合能力，促进细胞极性形成。

3. **"ARHGEF7重组蛋白的晶体结构解析及其自抑制机制"**

- 作者：Köhler K., et al.

- 摘要：通过X射线晶体学解析ARHGEF7重组蛋白的构象，揭示其C端PH结构域通过分子内相互作用抑制GEF活性，为靶向药物设计提供依据。

4. **"ARHGEF7重组蛋白在神经元突触发育中的功能验证"**

- 作者：Saneyoshi T., et al.

- 摘要：采用哺乳动物细胞表达ARHGEF7重组蛋白，证实其通过激活Rac1信号通路调控树突棘形态发生，影响突触可塑性。

(注：以上文献为模拟内容，实际文献需通过PubMed或SciHub等平台以关键词“ARHGEF7 recombinant”“beta-Pix GEF”检索。)

背景信息

ARHGEF7 (Rho Guanine Nucleotide Exchange Factor 7), also known as β-PIX, is a member of the RhoGEF family that regulates Rho GTPases, particularly Cdc42 and Rac1. These GTPases are critical for cytoskeletal reorganization, cell motility, and intracellular signaling. ARHGEF7 functions as a scaffold protein, coordinating signaling complexes through its multiple domains, including a DH (Dbl homology) domain responsible for GEF activity, a PH (pleckstrin homology) domain for membrane targeting, and SH3 (Src homology 3) domains mediating protein-protein interactions. It interacts with kinases like PAK (p21-activated kinase) to modulate cell adhesion, migration, and polarity.

Recombinant ARHGEF7 protein is typically produced in heterologous expression systems (e.g., E. coli, mammalian cells) to study its biochemical properties and cellular roles. Its production enables in vitro analysis of GEF activity, binding partners, and structural features. Researchers employ affinity tags (e.g., GST, His-tag) for purification, followed by tag removal and functional validation. The recombinant protein is vital for dissecting pathways in cancer metastasis, neuronal development, and immune responses, as ARHGEF7 dysregulation is linked to tumor invasion, synaptic plasticity defects, and immune cell trafficking. Its role in regulating focal adhesion dynamics also makes it relevant for studying mechanotransduction and tissue remodeling.