| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Rock1 |
| Uniprot No | Q13464 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-535aa |
| 氨基酸序列 | MSTGDSFETRFEKMDNLLRDPKSEVNSDCLLDGLDALVYDLDFPALRKNKNIDNFLSRYKDTINKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKSGHLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNDISKEAKNLICAFLTDREVRLGRNGVEEIKRHLFFKNDQWAWETLRDTVAPVVPDLSSDIDTSNFDDLEEDKGEEETFPIPKAFVGNQLPFVGFTYYSNRRYLSSANPNDNRTSSNADKSLQESLQKTIYKLEEQLHNEMQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRRNLESTVSQIEKEKMLLQHRINEYQRKAEQENEKRRNVENEVSTLKDQLEDLKKVSQNSQL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Rock1重组蛋白的3篇参考文献示例(文献为虚拟示例，仅供参考)：
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1. **文献名称**：*"Expression and functional characterization of recombinant ROCK1 kinase in cancer cell motility"*
**作者**：Li, X. et al.
**摘要**：本研究通过大肠杆菌表达系统成功纯化重组人源ROCK1蛋白，验证其激酶活性(对MYPT1底物的磷酸化)，并证明其在体外促进肿瘤细胞迁移的能力，为ROCK1靶向药物筛选提供基础工具。
2. **文献名称**：*"Structural insights into ROCK1 activation by RhoA binding using cryo-EM"*
**作者**：Wang, Y. et al.
**摘要**：通过冷冻电镜解析了RhoA结合的ROCK1重组蛋白三维结构，揭示了RhoA诱导的ROCK1构象变化及自抑制机制，为设计特异性抑制剂提供结构依据。
3. **文献名称**：*"ROCK1 regulates apoptosis in neuronal cells through recombinant protein-based assays"*
**作者**：Sato, M. et al.
**摘要**：利用昆虫细胞表达的重组ROCK1蛋白，结合体外激酶实验，证明ROCK1通过磷酸化Caspase-9调控神经元凋亡，提示其在神经退行性疾病中的潜在作用。
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*注：以上文献为模拟示例，实际引用需查询真实数据库(如PubMed)。建议使用关键词“ROCK1 recombinant protein”或“ROCK1 kinase assay”检索最新研究。*
**Background of ROCK1 Recombinant Protein**
ROCK1 (Rho-associated coiled-coil containing protein kinase 1) is a serine/threonine kinase belonging to the AGC family of protein kinases. It plays a critical role in regulating cytoskeletal dynamics, cell contraction, migration, and apoptosis through the Rho/ROCK signaling pathway. ROCK1 is activated by binding to the small GTPase RhoA (Ras homolog family member A), which triggers conformational changes, enabling the kinase to phosphorylate downstream targets such as myosin light chain (MLC), LIM kinase, and ezrin-radixin-moesin (ERM) proteins. These interactions modulate actin-myosin contractility, stress fiber formation, and cell adhesion, influencing processes like vascular tone, neuronal outgrowth, and cancer cell invasion.
The recombinant ROCK1 protein is engineered for research applications, typically produced using expression systems like *E. coli* or mammalian cells. Its purified form retains enzymatic activity, allowing *in vitro* studies to dissect ROCK1-mediated pathways or screen inhibitors. Structurally, ROCK1 contains an N-terminal kinase domain, a central coiled-coil region facilitating dimerization, and a C-terminal pleckstrin homology (PH) domain critical for RhoA binding. Dysregulation of ROCK1 is linked to cardiovascular diseases (e.g., hypertension, atherosclerosis), neurological disorders, fibrosis, and tumor metastasis, making it a therapeutic target.
ROCK1 recombinant protein is widely used in drug discovery, notably for developing inhibitors like Y-27632 and Fasudil, which show potential in treating glaucoma, pulmonary hypertension, and ischemic stroke. Its study also aids in understanding cellular mechanotransduction and tissue remodeling mechanisms. By leveraging recombinant ROCK1. researchers gain insights into its structure-function relationships and pathological contributions, advancing both basic science and clinical applications.
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