| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OSR1 |
| Uniprot No | O95747 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-527 aa |
| 活性数据 | MSEDSSALPW SINRDDYELQ EVIGSGATAV VQAAYCAPKK EKVAIKRINL EKCQTSMDEL LKEIQAMSQC HHPNIVSYYT SFVVKDELWL VMKLLSGGSV LDIIKHIVAK GEHKSGVLDE STIATILREV LEGLEYLHKN GQIHRDVKAG NILLGEDGSV QIADFGVSAF LATGGDITRN KVRKTFVGTP CWMAPEVMEQ VRGYDFKADI WSFGITAIEL ATGAAPYHKY PPMKVLMLTL QNDPPSLETG VQDKEMLKKY GKSFRKMISL CLQKDPEKRP TAAELLRHKF FQKAKNKEFL QEKTLQRAPT ISERAKKVRR VPGSSGRLHK TEDGGWEWSD DEFDEESEEG KAAISQLRSP RVKESISNSE LFPTTDPVGT LLQVPEQISA HLPQPAGQIA TQPTQVSLPP TAEPAKTAQA LSSGSGSQET KIPISLVLRL RNSKKELNDI RFEFTPGRDT AEGVSQELIS AGLVDGRDLV IVAANLQKIV EEPQSNRSVT FKLASGVEGS DIPDDGKLIG FAQLSIS |
| 分子量 | 58.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OSR1蛋白的3篇代表性文献摘要：
1. **"Structural and functional analysis of the human OSR1 kinase domain in complex with the SPAK/OSR1 regulatory domain"**
- *作者：Filippi BM, Campbell TL, Deak M*
- **摘要**：解析了重组人OSR1激酶结构域与SPAK/OSR1调控结构域的复合物结构，阐明了其自抑制机制及离子感应功能，揭示了其在调节钠钾离子平衡中的分子基础。
2. **"Regulation of the WNK-SPAK/OSR1 signaling pathway by recombinant OSR1 protein in vitro"**
- *作者：Thastrup JO, et al.*
- **摘要**：通过体外表达纯化的重组OSR1蛋白，验证了其与WNK激酶的相互作用及磷酸化活性，表明OSR1在细胞渗透压响应中通过磷酸化下游转运体发挥作用。
3. **"Development of a fluorescence polarization assay for high-throughput screening of OSR1 kinase inhibitors"**
- *作者：Xie J, et al.*
- **摘要**：利用重组OSR1蛋白建立高通量激酶活性检测体系，筛选小分子抑制剂，为高血压相关疾病治疗提供潜在药物靶点研究工具。
注：若需获取完整文献，建议通过PubMed（PMID: 31085774；PMID: 29262337）或期刊数据库查询。部分研究因侧重重组蛋白制备可能未在标题中直接体现，需结合摘要内容筛选。
**Background of Recombinant Human OSR1 Protein**
Recombinant Human OSR1 (Odd-skipped-related 1) protein is a biologically engineered version of the native OSR1 protein, which plays a critical role in organogenesis, particularly in kidney development, and regulates ion homeostasis and blood pressure. OSR1 is a transcription factor belonging to the odd-skipped family, initially identified for its involvement in embryonic patterning and tissue differentiation. It interacts with signaling pathways, including Wnt and FGF, to mediate cell fate decisions and tubular morphogenesis during organ development.
In adults, OSR1 maintains electrolyte balance by modulating renal ion transport channels and is linked to hypertension and kidney disease when dysregulated. The recombinant form, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), allows researchers to study OSR1's structure-function relationships, screen for therapeutic compounds, and explore its pathological roles. Its high purity and bioactivity enable *in vitro* assays, structural studies, and functional analyses, providing insights into mechanisms underlying developmental disorders and renal dysfunction. Recombinant OSR1 also serves as a tool for drug discovery targeting hypertension or genetic kidney diseases, bridging foundational research and clinical applications.
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