| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OSBPL3 |
| Uniprot No | Q9H4L5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-820 aa |
| 活性数据 | MMSDEKNLGVSQKLVSPSRSTSSCSSKQGSRQDSWEVVEGLRGEMNYTQEPPVQKGFLLKKRKWPLKGWHKRFFYLDKGILKYAKSQTDIEREKLHGCIDVGLSVMSVKKSSKCIDLDTEEHIYHLKVKSEEVFDEWVSKLRHHRMYRQNEIAMFPHEVNHFFSGSTITDSSSGVFDSISSRKRSSISKQNLFQTGSNVSFSCGGETRVPLWLQSSEDMEKCSKDLAHCHAYLVEMSQLLQSMDVLHRTYSAPAINAIQVPKPFSGPVRLHSSNPNLSTLDFGEEKNYSDGSETSSEFSKMQEDLCHIAHKVYFTLRSAFNIMSAEREKLKQLMEQDASSSPSAQVIGLKNALSSENSRDENRALVHQLSNESRLSITDSLSEFFDAQEVLLSPSSSENEISDDDSYVSDISDNLSLDNLSNDLDNERQTLGPVLDSGREAKSRRRTCLPAPCPSSSNISLWNILRNNIGKDLSKVAMPVELNEPLNTLQRLCEELEYSELLDKAAQIPSPLERMVYVAAFAISAYASSYYRAGSKPFNPVLGETYECIREDKGFQFFSEQVSHHPPISACHAESRNFVFWQDVRWKNKFWGKSMEIVPIGTTHVTLPVFGDHFEWNKVTSCIHNILSGQRWIEHYGEIVIKNLHDDSCYCKVNFIKAKYWSTNAHEIEGTVFDRSGKAVHRLFGKWHESIYCGGGSSSACVWRANPMPKGYEQYYSFTQFALELNEMDPSSKSLLPPTDTRFRPDQRFLEEGNLEEAEIQKQRIEQLQRERRRVLEENHVEHQPRFFRKSDDDSWVSNGTYLELRKDLGFSKLDHPVLW |
| 分子量 | 120.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设性示例，格式参考真实文献的结构，但具体内容可能需要通过学术数据库验证：
1. **文献名称**: "Recombinant human OSBPL3 facilitates cholesterol transport and promotes hepatocellular carcinoma progression"
**作者**: Chen CY, Wang L, Zhang X
**摘要**: 本研究通过在大肠杆菌中表达重组人OSBPL3蛋白，并分析其在肝癌细胞中的功能。结果表明，OSBPL3通过与胆固醇结合，促进肝癌细胞的脂质摄取及增殖，提示其作为治疗靶点的潜力。
2. **文献名称**: "OSBPL3 interacts with RAB7A to regulate endosomal trafficking via recombinant protein analysis"
**作者**: Kim TA, Park S, Lee JH
**摘要**: 利用重组OSBPL3蛋白进行体外相互作用实验，发现其与RAB7A直接结合，调控内体-溶酶体运输通路。研究揭示了OSBPL3在细胞内膜运输中的分子机制。
3. **文献名称**: "Structural insights into human OSBPL3 through X-ray crystallography of its recombinant form"
**作者**: Liu H, Zheng M, Wu Q
**摘要**: 本研究解析了重组OSBPL3蛋白的晶体结构，发现其氧固醇结合结构域的关键氨基酸残基，为理解其脂质结合功能提供结构基础。
**注意**：上述参考文献为示例，实际文献需通过PubMed、Google Scholar等数据库检索关键词如“OSBPL3 recombinant protein”或“OSBPL3 structure function”获取。建议结合具体研究方向筛选最新成果。
Recombinant human OSBPL3 (oxysterol-binding protein-like 3) is a protein encoded by the *OSBPL3* gene, which belongs to the oxysterol-binding protein (OSBP) family. This family is characterized by their ability to bind oxysterols, cholesterol derivatives involved in lipid metabolism and signaling. OSBPL3, also known as ORP3, contains conserved pleckstrin homology (PH) and FFAT (two phenylalanines in an acidic tract) domains, enabling interactions with membrane lipids and endoplasmic reticulum proteins. It plays roles in intracellular lipid transport, membrane trafficking, and cellular signaling pathways. Studies suggest OSBPL3 regulates actin cytoskeleton dynamics, cell adhesion, and migration, linking lipid metabolism to cellular architecture and motility. Dysregulation of OSBPL3 has been associated with cancer progression, neurodegenerative diseases, and metabolic disorders. Recombinant OSBPL3 is typically produced using expression systems like *E. coli* or mammalian cells, retaining functional domains for biochemical studies, protein-protein interaction assays, or lipid-binding analyses. Its recombinant form aids in elucidating molecular mechanisms in lipid homeostasis and disease pathogenesis, offering potential therapeutic insights.
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