| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ORMDL3 |
| Uniprot No | Q8N138 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-153 aa |
| 活性数据 | MNVGTAHSEVNPNTRVMNSRGIWLSYVLAIGLLHIVLLSIPFVSVPVVWTLTNLIHNMGM YIFLHTVKGTPFETPDQGKARLLTHWEQMDYGVQFTASRKFLTITPIVLYFLTSFYTKYD QIHFVLNTVSLMSVLIPKLPQLHGVRIFGINKY |
| 分子量 | 17.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ORMDL3蛋白的参考文献示例（注：以下内容为虚构，仅用于演示格式，实际文献请通过PubMed等数据库检索）：
1. **《ORMDL3 regulates ER-mediated calcium signaling and inflammatory response in asthma》**
- Miller, S. et al.
- 该研究揭示了ORMDL3通过调控内质网钙离子稳态，促进Th2炎症反应，证实其在哮喘发病机制中的关键作用。
2. **《Structural insights into ORMDL3 protein and its interaction with the serine palmitoyltransferase complex》**
- Zhang, Y. et al.
- 通过冷冻电镜解析ORMDL3的晶体结构，阐明其与丝氨酸棕榈酰转移酶（SPT）复合物的结合机制，为脂质代谢相关疾病提供分子基础。
3. **《ORMDL3 deficiency alters ER stress and enhances susceptibility to high-fat diet-induced obesity》**
- Chen, L. et al.
- 发现ORMDL3敲除小鼠内质网应激减弱，但高脂饮食下脂肪堆积加剧，提示其代谢调控的双重功能。
4. **《ORMDL3 promotes Th17 cell differentiation via modulating sphingolipid biosynthesis》**
- Kim, J. & Lee, H.
- 研究表明ORMDL3通过调节鞘脂合成通路促进Th17细胞分化，关联自身免疫性疾病的发展。
建议使用关键词"ORMDL3 recombinant protein"或"ORMDL3 function"在PubMed或Google Scholar中检索最新文献以获取真实数据。
ORMDL3, a member of the ORMDL (orosomucoid-like) protein family, is an endoplasmic reticulum (ER)-transmembrane protein encoded by the *ORMDL3* gene in humans. It regulates sphingolipid biosynthesis by inhibiting serine palmitoyltransferase (SPT), the rate-limiting enzyme in de novo sphingolipid production, thereby maintaining lipid homeostasis. ORMDL3 also modulates ER stress responses, calcium signaling, and the unfolded protein response (UPR), linking it to cellular stress adaptation mechanisms.
Genome-wide association studies (GWAS) have identified *ORMDL3* polymorphisms as significant risk factors for asthma and other inflammatory diseases. Elevated ORMDL3 expression correlates with airway remodeling, cytokine release, and immune dysregulation, suggesting a role in asthma pathogenesis. Additionally, it has been implicated in type 1 diabetes, inflammatory bowel disease, and cancer, highlighting its broad regulatory influence.
Recombinant human ORMDL3 protein is engineered for in vitro studies to dissect its molecular interactions, structural features, and disease-related mechanisms. Produced via bacterial or mammalian expression systems, it serves as a tool for screening therapeutic agents targeting ORMDL3-associated pathways. Research on recombinant ORMDL3 continues to unveil its dual roles in lipid metabolism and inflammation, offering potential insights into precision therapies for chronic inflammatory disorders.
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