| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ORC6L |
| Uniprot No | Q9Y5N6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-252 aa |
| 活性数据 | MGSELIGRLAPRLGLAEPDMLRKAEEYLRLSRVKCVGLSARTTETSSAVMCLDLAASWMKCPLDRAYLIKLSGLNKETYQSCLKSFECLLGLNSNIGIRDLAVQFSCIEAVNMASKILKSYESSLPQTQQVDLDLSRPLFTSAALLSACKILKLKVDKNKMVATSGVKKAIFDRLCKQLEKIGQQVDREPGDVATPPRKRKKIVVEAPAKEMEKVEEMPHKPQKDEDLTQDYEEWKRKILENAASAQKATAE |
| 分子量 | 55.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ORC6L蛋白的3篇参考文献示例（文献为模拟概括，供参考）：
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1. **文献名称**：*Structural insights into the human origin recognition complex subunit ORC6L*
**作者**：Prasanth, S.G. et al.
**摘要**：通过重组表达和纯化人ORC6L蛋白，结合冷冻电镜技术解析其三维结构，揭示其与ORC复合体其他亚基（如ORC2/3）的相互作用界面，并发现其C端结构域对DNA复制起始的调控作用。
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2. **文献名称**：*Functional analysis of ORC6L in DNA replication initiation using recombinant protein assays*
**作者**：Chesnokov, I., Remus, D.
**摘要**：通过体外重组表达ORC6L蛋白，证明其在识别复制起点中的必要性，并发现其与CDC6和MCM复合体的相互作用，揭示了ORC6L在复制前复合体组装中的关键衔接功能。
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3. **文献名称**：*ORC6L mutations in Meier-Gorlin syndrome disrupt DNA replication licensing*
**作者**：Bicknell, L.S. et al.
**摘要**：利用重组野生型与突变型ORC6L蛋白进行功能互补实验，发现疾病相关突变（如R105Q）削弱ORC复合体稳定性，导致细胞周期阻滞和复制起始缺陷，首次将ORC6L突变与侏儒症表型关联。
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**注**：以上文献为示例性概括，具体研究请参考真实数据库（如PubMed）中的论文。如需精准文献，建议提供更具体的研究方向（如结构、疾病或相互作用）。
The origin recognition complex (ORC) is a conserved multi-protein assembly essential for initiating DNA replication in eukaryotic cells. As a key subunit, ORC6 (ORC6L in humans) plays a critical role in coordinating the ORC’s function during the G1 phase of the cell cycle. Structurally, ORC6L is the smallest subunit (28 kDa) and exhibits species-specific functional divergence. While its N-terminal domain interacts with ORC3 to stabilize the complex, the C-terminal domain facilitates replication origin binding in metazoans. Unlike other ORC subunits, ORC6L also participates in cytokinesis and chromosomal segregation independently of its replication role, highlighting its multifunctional nature.
Recombinant human ORC6L protein, typically expressed in bacterial or mammalian systems, enables biochemical and structural studies to dissect its mechanisms. Its production supports research on replication origin licensing, particularly in conjunction with CDC6 and MCM complexes. Mutations in ORC6L are linked to Meier-Gorlin syndrome, a genetic disorder characterized by impaired growth and microcephaly, emphasizing its biological significance. Current studies focus on ORC6L’s role in cancer, where replication dysregulation drives genomic instability, and its potential as a therapeutic target. Structural insights from recombinant ORC6L could inform drug design to modulate replication processes in proliferative diseases.
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