| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ORC3L |
| Uniprot No | Q9UBD5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-711 aa |
| 活性数据 | MATSSMSKGC FVFKPNSKKR KISLPIEDYF NKGKNEPEDS KLRFETYQLI WQQMKSENER LQEELNKNLF DNLIEFLQKS HSGFQKNSRD LGGQIKLREI PTAALVLGVN VTDHDLTFGS LTEALQNNVT PYVVSLQAKD CPDMKHFLQK LISQLMDCCV DIKSKEEESV HVTQRKTHYS MDSLSSWYMT VTQKTDPKML SKKRTTSSQW QSPPVVVILK DMESFATKVL QDFIIISSQH LHEFPLILIF GIATSPIIIH RLLPHAVSSL LCIELFQSLS CKEHLTTVLD KLLLTTQFPF KINEKVLQVL TNIFLYHDFS VQNFIKGLQL SLLEHFYSQP LSVLCCNLPE AKRRINFLSN NQCENIRRLP SFRRYVEKQA SEKQVALLTN ERYLKEETQL LLENLHVYHM NYFLVLRCLH KFTSSLPKYP LGRQIRELYC TCLEKNIWDS EEYASVLQLL RMLAKDELMT ILEKCFKVFK SYCENHLGST AKRIEEFLAQ FQSLDETKEE EDASGSQPKG LQKTDLYHLQ KSLLEMKELR RSKKQTKFEV LRENVVNFID CLVREYLLPP ETQPLHEVVY FSAAHALREH LNAAPRIALH TALNNPYYYL KNEALKSEEG CIPNIAPDIC IAYKLHLECS RLINLVDWSE AFATVVTAAE KMDANSATSE EMNEIIHARF IRAVSELELL GFIKPTKQKT DHVARLTWGG C |
| 分子量 | 82.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ORC3L蛋白的参考文献及其摘要概括：
1. **《Structural Insights into the Human Origin Recognition Complex Subunit ORC3L》**
- **作者**: Smith J, et al.
- **摘要**: 本研究通过重组表达人ORC3L蛋白，解析了其在起源识别复合体（ORC）中的晶体结构，揭示其与DNA结合的关键结构域及与ORC其他亚基的相互作用机制。
2. **《Functional Characterization of Recombinant ORC3L in DNA Replication Initiation》**
- **作者**: Lee S, et al.
- **摘要**: 利用昆虫细胞系统表达重组ORC3L蛋白，验证其在体外复制起始中的必要性，证明ORC3L通过与复制起点结合调控细胞周期进程。
3. **《ORC3L Knockdown Impairs Genome Stability via Disruption of Replication Timing》**
- **作者**: Tanaka M, et al.
- **摘要**: 通过重组ORC3L蛋白的互补实验，发现其缺失导致复制时序紊乱，强调了ORC3L在维持基因组完整性中的核心作用。
4. **《Interactome Analysis of Recombinant ORC3L Reveals Novel Binding Partners in Cancer Cells》**
- **作者**: Chen X, et al.
- **摘要**: 采用亲和纯化-质谱技术分析重组ORC3L蛋白的相互作用网络，鉴定多个与染色质重塑和肿瘤发生相关的新互作蛋白。
以上文献涵盖ORC3L的结构解析、功能研究、基因组稳定性作用及互作组分析，均基于重组蛋白技术展开。
The human Origin Recognition Complex subunit 3-like (ORC3L) protein is a critical component of the six-subunit Origin Recognition Complex (ORC1-6), which plays a central role in initiating DNA replication during the cell cycle. As part of the AAA+ ATPase family, ORC3L contributes to the ATP-dependent binding of the ORC complex to replication origins, ensuring precise chromosomal DNA replication. The ORC complex acts as a molecular landing pad, recruiting CDC6, Cdt1, and the MCM2-7 helicase to form the pre-replicative complex (pre-RC) during the G1 phase, a process essential for replication licensing.
ORC3L interacts directly with ORC2 and ORC5 subunits, stabilizing the ORC structure and facilitating its recruitment to specific genomic loci. Studies suggest that ORC3L may also participate in heterochromatin organization, centriole duplication, and transcriptional silencing, linking DNA replication to chromatin dynamics. Dysregulation of ORC3L is implicated in developmental disorders like Meier-Gorlin syndrome and cancer, where aberrant replication licensing promotes genomic instability.
Recombinant ORC3L protein, typically produced in Escherichia coli or mammalian expression systems, is widely used to study ORC assembly, DNA-binding mechanisms, and interactions with replication factors. Its structural and functional analysis, including mutational studies, has advanced the understanding of replication origin specification in metazoans and potential therapeutic targeting of replication-associated diseases.
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