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Recombinant Human ORC5L Protein

  • 中文名: 重组人(ORC5L)蛋白
  • 别    名: ORC5; ORC5_HUMAN; ORC5L; ORC5P; ORC5T; Origin recognition complex subunit 5; Origin recognition complex subunit 5 like
货号: PAX2000-10043
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点ORC5L
Uniprot NoO43913
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-435 aa
活性数据MPHLENVVLC RESQVSILQS LFGERHHFSF PSIFIYGHTA SGKTYVTQTL LKTLELPHVF VNCVECFTLR LLLEQILNKL NHLSSSEDGC STEITCETFN DFVRLFKQVT TAENLKDQTV YIVLDKAEYL RDMEANLLPG FLRLQELADR NVTVLFLSEI VWEKFRPNTG CFEPFVLYFP DYSIGNLQKI LSHDHPPEYS ADFYAAYINI LLGVFYTVCR DLKELRHLAV LNFPKYCEPV VKGEASERDT RKLWRNIEPH LKKAMQTVYL REISSSQWEK LQKDDTDPGQ LKGLSAHTHV ELPYYSKFIL IAAYLASYNP ARTDKRFFLK HHGKIKKTNF LKKHEKTSNH LLGPKPFPLD RLLAILYSIV DSRVAPTANI FSQITSLVTL QLLTLVGHDD QLDGPKYKCT VSLDFIRAIA RTVNFDIIKY LYDFL
分子量50.2 kDa
蛋白标签His tag N-Terminus
缓冲液0
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.


参考文献



以下是关于重组人ORC5L蛋白的3条参考文献(基于公开研究信息概括,非真实文献,供参考):


1. **"Structural insights into the human origin recognition complex subunit ORC5L"**  

   *作者:Bleichert F. et al. (2017)*  

   摘要:通过重组表达的ORC5L蛋白结合冷冻电镜技术,揭示了其在ORC复合体中的结构作用,发现ORC5L对复合体与DNA结合的关键功能,并探讨了其突变对复制起始的影响。


2. **"Functional analysis of ORC5L in DNA replication initiation using recombinant human proteins"**  

   *作者:Noguchi Y. et al. (2015)*  

   摘要:研究利用重组表达的ORC5L与其他亚基重构ORC复合体,证明其协同参与复制起点的识别和启动,并通过体外实验验证ORC5L缺失导致复制活性丧失。


3. **"ORC5L mutations disrupt chromatin binding and are linked to Meier-Gorlin syndrome"**  

   *作者:de Munnik S.A. et al. (2012)*  

   摘要:通过重组ORC5L蛋白的功能实验,发现其突变会破坏ORC复合体与染色质的结合,阐明了ORC5L缺陷导致人类发育异常(如Meier-Gorlin综合征)的分子机制。


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*注:以上文献为模拟示例,实际研究中请结合具体数据库(如PubMed)验证。若需真实文献,可检索关键词“ORC5L recombinant protein”或“ORC5L DNA replication”进一步筛选。*


背景信息



The origin recognition complex subunit 5-like (ORC5L) protein is a critical component of the hexameric origin recognition complex (ORC) in eukaryotes, which plays a central role in initiating DNA replication during the cell cycle. ORC binds to replication origins and serves as a platform for assembling the pre-replication complex, ensuring precise genome duplication. As the fifth subunit, ORC5L contributes to the structural stability of the ORC complex and participates in chromatin binding, though its specific functional mechanisms remain less characterized compared to other ORC subunits. Studies suggest ORC5L interacts with ORC2 and ORC3, forming a core subcomplex essential for coordinating replication initiation.


Recombinant human ORC5L is typically produced using heterologous expression systems (e.g., E. coli or insect cells) for biochemical and structural studies. Its recombinant form enables researchers to investigate ORC assembly dynamics, DNA-binding properties, and mutations linked to developmental disorders like Meier-Gorlin syndrome, which is associated with ORC subunit deficiencies. Recent work also explores ORC5L's potential regulatory roles beyond replication, including transcriptional control and chromatin remodeling. However, challenges persist in purifying full-length ORC5L due to its insolubility, prompting the use of truncated variants for functional analyses. Ongoing research aims to elucidate its contribution to replication fidelity and cell cycle regulation in health and disease.


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