| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ORC1L |
| Uniprot No | Q13415 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-110 aa |
| 活性数据 | MAHYPTRLKTRKTYSWVGRPLLDRKLHYQTYREMCVKTEGCSTEIHIQIGQFVLIEGDDDENPYVAKLLELFEDDSDPPPKKRARVQWFVRFCEVPACKRHLLGRKPGAQ |
| 分子量 | 37.73 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ORC1L蛋白的3篇参考文献示例（基于公开研究整理，具体文献需通过学术数据库核实）：
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1. **文献名称**：**"The human origin recognition complex is essential for DNA replication"**
**作者**：Chesnokov, I., et al.
**摘要**：该研究解析了人源ORC复合体（含ORC1L亚基）在DNA复制起始中的核心作用，证明重组ORC1L蛋白与其他亚基结合后能够识别复制起点，并在体外实验中启动复制活性。
2. **文献名称**：**"Structure and function of the Orc1 BAH domain required for heterochromatin association"**
**作者**：Zhang, Y., et al.
**摘要**：通过重组表达ORC1L蛋白的BAH结构域，结合晶体结构分析和体外结合实验，揭示其与组蛋白H4K20me2修饰的相互作用，并阐述其在染色质沉默和复制中的功能关联。
3. **文献名称**：**"Mutations in ORC1L cause Meier-Gorlin syndrome, a disorder of DNA replication"**
**作者**：Bicknell, L.S., et al.
**摘要**：研究发现ORC1L基因突变导致Meier-Gorlin综合征（侏儒症伴小耳畸形），通过重组蛋白功能分析表明突变体显著降低ORC复合体的稳定性，影响细胞周期进程。
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**备注**：以上文献信息为示例，具体引用时建议通过PubMed或Google Scholar使用关键词（如ORC1L、recombinant protein）检索原文，并核对作者及出版年份。
The Origin Recognition Complex Subunit 1-Like (ORC1L) protein is a critical component of the hexameric Origin Recognition Complex (ORC) in eukaryotes, which plays a central role in initiating DNA replication. As the largest subunit of ORC, ORC1L is essential for recognizing and binding replication origins, the specific genomic sites where replication begins. It facilitates the assembly of the pre-replication complex (pre-RC) by recruiting key factors like CDC6 and the MCM helicase complex, ensuring proper licensing of replication origins during the G1 phase of the cell cycle. Structurally, ORC1L contains an ATP-binding AAA+ domain, which is crucial for its ATPase activity and interaction with other replication proteins.
Beyond replication initiation, ORC1L participates in chromatin organization, gene silencing, and centrosome duplication. Dysregulation or mutations in ORC1L are linked to developmental disorders such as Meier-Gorlin syndrome, characterized by growth defects and microcephaly, and are implicated in cancers due to genomic instability. Recombinant ORC1L protein, often produced using bacterial or eukaryotic expression systems, enables mechanistic studies of replication origin firing, structural analyses (e.g., cryo-EM), and drug discovery targeting replication anomalies. Its purified form also aids in exploring protein-protein/DNA interactions, providing insights into replication licensing checkpoints and cell cycle regulation. This makes ORC1L a vital tool for understanding fundamental biological processes and developing therapeutic strategies.
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