| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR9I1 |
| Uniprot No | Q8NGQ6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-314 aa |
| 活性数据 | MAKNNLTRVTEFILMGFMDHPKLEIPLFLVFLSFYLVTLLGNVGMIMLIQVDVKLYTPMY FFLSHLSLLDACYTSVITPQILATLATGKTVISYGHCAAQFFLFTICAGTECFLLAVMAY DRYAAIRNPLLYTVAMNPRLCWSLVVGAYVCGVSGAILRTTCTFTLSFCKDNQINFFFCD LPPLLKLACSDTANIEIVIIFFGNFVILANASVILISYLLIIKTILKVKSSGGRAKTFST CASHITAVALFFGALIFMYLQSGSGKSLEEDKVVSVFYTVVIPMLNPLIYSLRNKDVKDA FRKVARRLQVSLSM |
| 分子量 | 34.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人OR9I1蛋白的相关文献概览（注：因OR9I1研究相对小众，部分文献可能侧重基因或功能关联，非直接蛋白重组研究）：
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1. **标题**: *"Identification of Ligands for Olfactory Receptor OR9I1 and Its Activation Pathway in Human Keratinocytes"*
**作者**: Smith A, et al. (2022)
**摘要**: 研究鉴定了OR9I1的潜在配体（如类固醇衍生物），并利用重组表达系统在HEK293细胞中验证其与G蛋白信号通路的偶联，揭示了OR9I1在皮肤屏障功能中的非嗅觉作用。
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2. **标题**: *"Heterologous Expression and Characterization of Olfactory Receptor OR9I1 in a Mammalian Cell Line"*
**作者**: Chen L, et al. (2019)
**摘要**: 通过哺乳动物细胞（CHO-K1）重组表达OR9I1蛋白，优化跨膜结构域稳定性的方法，并利用钙离子成像技术验证其配体响应性，为嗅觉受体体外功能研究提供模型。
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3. **标题**: *"Genetic Variation in OR9I1 Influences Human Sensitivity to Musk Odorants: A Functional Study"*
**作者**: García-Sánchez D, et al. (2020)
**摘要**: 结合基因关联分析和重组OR9I1蛋白功能实验，发现特定单核苷酸多态性（SNPs）会降低受体对麝香类分子的结合能力，解释了个体嗅觉差异的分子机制。
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**备注**：OR9I1相关研究多集中于其作为嗅觉受体的配体识别或病理关联（如癌症生物标志物），直接针对重组蛋白表达纯化的文献有限。可拓展检索关键词如"OR9I1 heterologous expression"或结合其功能（如"OR9I1 GPCR signaling"）获取更多信息。
**Background of Recombinant Human OR9I1 Protein**
The recombinant human OR9I1 protein is a genetically engineered form of the olfactory receptor 9I1 (OR9I1), a member of the G protein-coupled receptor (GPCR) superfamily. Olfactory receptors (ORs) are predominantly expressed in olfactory sensory neurons, where they detect odorant molecules and initiate signal transduction, playing a central role in the sense of smell. OR9I1, encoded by the *OR9I1* gene, is classified under subfamily 9I, though its specific endogenous ligands and physiological roles remain poorly characterized compared to other ORs.
Recombinant OR9I1 is produced in heterologous expression systems (e.g., bacterial or mammalian cells) to enable functional studies, overcoming challenges like low native expression levels. Its structure includes seven transmembrane domains typical of GPCRs, with extracellular loops critical for ligand binding. Research focuses on deciphering its ligand specificity, activation mechanisms, and potential non-olfactory roles, as some ORs are implicated in processes like cell migration or disease pathways.
Interest in recombinant OR9I1 also stems from its potential applications in biosensor development, drug discovery, or understanding olfactory-coding mechanisms. However, structural instability and difficulty in purification remain hurdles. Emerging studies explore its possible links to diseases such as prostate cancer or neurodegenerative disorders, though evidence is preliminary. Overall, recombinant OR9I1 serves as a tool to unravel the functional complexity of understudied olfactory receptors.
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