| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PAAF1 |
| Uniprot No | Q9BRP4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-392 aa |
| 活性数据 | AAPLRIQSD WAQALRKDEG EAWLSCHPPG KPSLYGSLTC QGIGLDGIPE VTASEGFTVN EINKKSIHIS CPKENASSKF LAPYTTFSRI HTKSITCLDI SSRGGLGVSS STDGTMKIWQ ASNGELRRVL EGHVFDVNCC RFFPSGLVVL SGGMDAQLKI WSAEDASCVV TFKGHKGGIL DTAIVDRGRN VVSASRDGTA RLWDCGRSAC LGVLADCGSS INGVAVGAAD NSINLGSPEQ MPSEREVGTE AKMLLLARED KKLQCLGLQS RQLVFLFIGS DAFNCCTFLS GFLLLAGTQD GNIYQLDVRS PRAPVQVIHR SGAPVLSLLS VRDGFIASQG DGSCFIVQQD LDYVTELTGA DCDPVYKVAT WEKQIYTCCR DGLVRRYQLS DL |
| 分子量 | 42.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人PAAF1（Proteasomal ATPase-Associated Factor 1）蛋白的参考文献概要，涵盖其在蛋白酶体功能及疾病中的研究：
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1. **文献名称**：*"Recombinant human PAAF1 modulates 26S proteasome assembly and cellular stress response"*
**作者**：Chen L. et al.
**摘要**：该研究利用重组人PAAF1蛋白体外实验，发现其通过与蛋白酶体ATP酶亚基相互作用，调控26S蛋白酶体的组装，并影响细胞在氧化应激条件下的蛋白降解能力，表明PAAF1在应激应答中具有关键作用。
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2. **文献名称**：*"Structural characterization of PAAF1 and its role in cancer cell proliferation"*
**作者**：Kimura S. et al.
**摘要**：作者解析了重组人PAAF1的晶体结构，发现其C端结构域介导与蛋白酶体的结合。功能实验显示，PAAF1过表达促进乳腺癌细胞的增殖，提示其可能作为肿瘤治疗的潜在靶点。
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3. **文献名称**：*"PAF1/PAAAF1 deficiency impairs mitochondrial function via proteasome dysregulation"*
**作者**：Rodríguez-Muela N. et al.
**摘要**：本研究通过敲低PAAF1表达并结合重组蛋白回补实验，证明PAAF1缺失导致蛋白酶体活性下降，进而引发线粒体功能障碍和神经元退行性变，提示其在神经退行性疾病中的病理机制。
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注：上述文献为虚构示例，实际研究中建议通过PubMed或Web of Science以关键词“recombinant PAAF1”、“PAF1 proteasome”进一步检索。
**Background of Recombinant Human PAAF1 Protein**
Proteasome-Associated Antioxidant Factor 1 (PAAF1), also known as PAAF or C16orf35, is a nuclear-encoded protein linked to proteasome function and cellular stress responses. Structurally, PAAF1 contains a conserved N-terminal domain and a C-terminal region critical for binding to the 26S proteasome, a multi-subunit complex responsible for degrading ubiquitinated proteins. Studies suggest PAAF1 acts as a regulatory component, modulating proteasome activity under oxidative stress or DNA damage, possibly by influencing substrate recognition or proteasomal assembly.
Recombinant human PAAF1 protein is engineered using molecular cloning techniques, typically expressed in *E. coli* or mammalian systems to ensure proper folding and post-translational modifications. The recombinant form retains functional domains, enabling research into its role in proteostasis, cell cycle regulation, and apoptosis. Dysregulation of PAAF1 has been implicated in pathological conditions, including neurodegenerative diseases and cancer, where proteasome dysfunction contributes to protein aggregation or uncontrolled proliferation. Current studies leverage recombinant PAAF1 to elucidate mechanisms of proteasome regulation, screen therapeutic compounds, and explore its potential as a biomarker. This protein thus serves as a vital tool for advancing understanding of cellular quality control and stress adaptation pathways.
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