| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Raftlin |
| Uniprot No | Q14699 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-578aa |
| 氨基酸序列 | GCGLNKLEK RDEKRPGNIY STLKRPQVET KIDVSYEYRF LEFTTLSAAE LPGSSAVRLA SLRDLPAQLL ELYQQGFSLA ALHPFVQPTH EREKTPLEHI FRAILIKKTD RSQKTDLHNE GYILELDCCS SLDHPTDQKL IPEFIKKIQE AASQGLKFVG VIPQYHSSVN SAGSSAPVST ANSTEDARDA KNARGDHASL ENEKPGTGDV CSAPAGRNQS PEPSSGPRGE VPLAKQPSSP SGEGDGGELS PQGVSKTLDG PESNPLEVHE EPLSGKMEIF TLFNKPKSHQ KCRQYYPVTI PLHVSKNGQT VSGLDANWLE HMSDHFRKGG MLVNAVFYLG IVNDSLHGLT DGVFIFEAVS TEDSKTIQGY DAIVVEQWTV LEGVEVQTDY VPLLNSLAAY GWQLTCVLPT PVVKTTSEGS VSTKQIVFLQ RPCLPQKIKK KESKFQWRFS REEMHNRQMR KSKGKLSARD KQQAEENEKN LEDQSSKAGD MGNCVSGQQQ EGGVSEEMKG PVQEDKGEQL SPGGLLCGVG VEGEAVQNGP ASHSRALVGI CTGHSNPGED ARDGDAEEVR ELGTVEEN |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Raftlin重组蛋白的3篇代表性文献摘要(虚构示例，仅供参考实际研究主题方向)：
1. **文献名称**: *Raftlin is required for lipid raft organization and Toll-like receptor signaling in B cells*
**作者**: Takeshita et al.
**摘要**: 研究发现Raftlin蛋白通过维持B细胞脂筏结构完整性，促进TLR4信号通路的激活，从而调控炎症因子(如TNF-α、IL-6)的分泌，揭示其在先天免疫中的关键作用。
2. **文献名称**: *Recombinant Raftlin exacerbates intestinal inflammation by enhancing macrophage infiltration in IBD models*
**作者**: Nakamura et al.
**摘要**: 通过重组Raftlin蛋白处理实验性结肠炎小鼠，发现其通过激活NF-κB通路加剧巨噬细胞向肠道黏膜的迁移，提示Raftlin可能作为炎症性肠病的潜在治疗靶点。
3. **文献名称**: *Raftlin expression correlates with disease severity in rheumatoid arthritis and is epigenetically regulated*
**作者**: Lee & Park
**摘要**: 分析类风湿关节炎患者滑膜组织，发现Raftlin蛋白表达水平与关节破坏程度正相关，其启动子区DNA甲基化状态影响表达量，为自身免疫疾病机制提供新视角。
(注：以上文献为模拟示例，实际研究请通过PubMed或Google Scholar检索关键词如"Raftlin protein"、"RFTN1 immune"等。)
**Background of Raftlin Recombinant Protein**
Raftlin, short for "Raftlin, Lymphocyte Raft Protein," is a conserved eukaryotic protein initially identified as a critical component of lipid rafts in lymphocytes. Lipid rafts are cholesterol- and sphingolipid-rich microdomains in cell membranes, playing essential roles in signal transduction, membrane trafficking, and immune cell activation. Raftlin is highly expressed in immune cells, particularly B and T lymphocytes, and is implicated in organizing membrane raft structures to facilitate receptor clustering and signaling.
Studies suggest Raftlin contributes to immune regulation by modulating B-cell receptor (BCR) signaling and Toll-like receptor (TLR) pathways. Its absence or dysfunction has been linked to impaired immune responses, highlighting its role in maintaining cellular communication and antigen presentation. Additionally, Raftlin is found in non-lymphoid tissues, such as the intestinal epithelium, where it may influence barrier function and inflammatory processes.
The recombinant Raftlin protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional forms for research. This allows scientists to study its structural properties, interactions with lipid rafts, and mechanistic roles in diseases like autoimmune disorders, cancers, and infections. Recombinant Raftlin also serves as a tool to explore therapeutic strategies, such as targeting lipid raft-associated pathways or developing biomarkers for immune dysregulation.
Current research focuses on elucidating Raftlin's molecular interactions, post-translational modifications, and tissue-specific functions, aiming to uncover its potential as a diagnostic or therapeutic target in immune-related pathologies.
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