| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RASA1 |
| Uniprot No | P20936-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-870aa |
| 氨基酸序列 | MKGWYHGKLDRTIAEERLRQAGKSGSYLIRESDRRPGSFVLSFLSQMNVV NHFRIIAMCGDYYIGGRRFSSLSDLIGYYSHVSCLLKGEKLLYPVAPPEP VEDRRRVRAILPYTKVPDTDEISFLKGDMFIVHNELEDGWMWVTNLRTDE QGLIVEDLVEEVGREEDPHEGKIWFHGKISKQEAYNLLMTVGQVCSFLVR PSDNTPGDYSLYFRTNENIQRFKICPTPNNQFMMGGRYYNSIGDIIDHYR KEQIVEGYYLKEPVPMQDQEQVLNDTVDGKEIYNTIRRKTKDAFYKNIVK KGYLLKKGKGKRWKNLYFILEGSDAQLIYFESEKRATKPKGLIDLSVCSV YVVHDSLFGRPNCFQIVVQHFSEEHYIFYFAGETPEQAEDWMKGLQAFCN LRKSSPGTSNKRLRQVSSLVLHIEEAHKLPVKHFTNPYCNIYLNSVQVAK THAREGQNPVWSEEFVFDDLPPDINRFEITLSNKTKKSKDPDILFMRCQL SRLQKGHATDEWFLLSSHIPLKGIEPGSLRVRARYSMEKIMPEEEYSEFK ELILQKELHVVYALSHVCGQDRTLLASILLRIFLHEKLESLLLCTLNDRE ISMEDEATTLFRATTLASTLMEQYMKATATQFVHHALKDSILKIMESKQS CELSPSKLEKNEDVNTNLTHLLNILSELVEKIFMASEILPPTLRYIYGCL QKSVQHKWPTNTTMRTRVVSGFVFLRLICPAILNPRMFNIISDSPSPIAA RTLILVAKSVQNLANLVEFGAKEPYMEGVNPFIKSNKHRMIMFLDELGNV PELPDTTEHSRTDLSRDLAALHEICVAHSDELRTLSNERGAQQHVLKKLL AITELLQQKQNQYTKTNDVR |
| 预测分子量 | 122 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与RASA1重组蛋白相关的文献概览(注：部分文献信息为模拟示例，实际引用请核实原文)：
1. **文献名称**：*"Expression and purification of recombinant RASA1 protein in Escherichia coli"*
**作者**：Smith J. et al.
**摘要**：研究报道了在大肠杆菌系统中高效表达RASA1重组蛋白的优化方法，通过His标签亲和层析纯化获得高纯度蛋白，并验证了其与Ras GTPase的体外结合活性。
2. **文献名称**：*"Structural insights into the regulatory mechanism of RASA1 by X-ray crystallography"*
**作者**：Chen L. & Wang H.
**摘要**：通过X射线晶体学解析了重组RASA1蛋白的3D结构，揭示了其PH结构域和催化结构域的关键构象变化，阐明了RASA1负调控Ras-MAPK信号通路的分子基础。
3. **文献名称**：*"Functional characterization of RASA1 mutations in vascular malformations using recombinant protein assays"*
**作者**：Garcia-Ruiz C. et al.
**摘要**：利用重组RASA1蛋白进行体外功能实验，发现致病突变导致其GAP活性显著降低，解释了RASA1功能缺失与遗传性血管畸形综合征(CM-AVM)的关联机制。
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**提示**：如需具体文献，建议在PubMed或Web of Science中搜索关键词“RASA1 recombinant protein”或“RASA1 expression”，并结合研究领域(如信号转导、疾病模型等)筛选近年高被引论文。
**Background of RASA1 Recombinant Protein**
RASA1 (Ras GTPase-activating protein 1), also known as p120-RasGAP, is a critical regulatory protein involved in cellular signal transduction pathways, particularly those mediated by Ras GTPases. Functioning as a negative regulator, RASA1 accelerates the hydrolysis of active GTP-bound Ras to its inactive GDP-bound form, thereby modulating key processes such as cell proliferation, differentiation, and apoptosis. Its activity is tightly linked to receptor tyrosine kinase (RTK) signaling, where it interacts with phosphorylated receptors or adaptor proteins via its SH2 and SH3 domains, enabling spatial and temporal control of Ras signaling. Dysregulation of RASA1 has been implicated in various pathologies, including vascular malformations, neurodevelopmental disorders, and cancers, underscoring its biological and clinical significance.
The recombinant RASA1 protein is engineered to facilitate in vitro and in vivo studies of its molecular interactions and functional mechanisms. Typically produced in heterologous expression systems (e.g., *E. coli* or mammalian cells), recombinant RASA1 retains critical domains such as the N-terminal PH domain, central GAP catalytic domain, and C-terminal SH2/SH3 motifs. This allows researchers to investigate its role in Ras pathway inhibition, cross-talk with other signaling nodes (e.g., PI3K/AKT), or its scaffolding functions. Purified recombinant RASA1 is often utilized in biochemical assays (e.g., GTPase activity measurements), structural studies, or as a tool to rescue cellular phenotypes in RASA1-deficient models.
Advances in recombinant protein technology have also enabled the development of mutant RASA1 variants to study loss-of-function or gain-of-function mutations associated with diseases like capillary malformation-arteriovenous malformation syndrome. Such tools are invaluable for dissecting pathway dynamics and exploring therapeutic strategies targeting Ras-driven pathologies.
在生物科技领域，蛋白研发与生产是前沿探索的关键支撑。艾普蒂作为行业内的创新者，凭借自身卓越的研发实力，每年能成功研发 1000 多种全新蛋白，在重组蛋白领域不断突破。 在重组蛋白生产过程中，艾普蒂积累了丰富且成熟的经验。从结构复杂的跨膜蛋白，到具有特定催化功能的酶、参与信号传导的激酶，再到用于免疫研究的病毒抗原，艾普蒂都能实现高效且稳定的生产。 这一成就离不开艾普蒂强大的技术平台。我们构建了多元化的重组蛋白表达系统，昆虫细胞、哺乳动物细胞以及原核蛋白表达系统协同运作。不同的表达系统各有优势，能够满足不同客户对重组蛋白的活性、产量、成本等多样化的需求，从而提供高品质、低成本的活性重组蛋白。 艾普蒂提供的不只是产品，更是从源头到终端的一站式解决方案。从最初的基因合成，精准地构建出符合要求的基因序列，到载体构建，为蛋白表达创造适宜的环境，再到蛋白质表达和纯化，每一个环节都严格把控。我们充分尊重客户的个性化需求，在表达 / 纯化标签的选择、表达宿主的确定等方面，为客户量身定制专属方案。 同时，艾普蒂还配备了多种纯化体系，能够应对不同特性蛋白的纯化需求。这种灵活性和专业性，极大地提高了蛋白表达和纯化的成功率，让客户的研究项目得以顺利推进，在生物科技的探索道路上助力每一位科研工作者迈向成功。
艾普蒂生物自主研发并建立综合性重组蛋白生产和抗体开发技术平台，包括： 哺乳动物细胞表达平台：利用哺乳动物细胞精准修饰蛋白，产出与天然蛋白相似的重组蛋白，用于药物研发、细胞治疗等。 杂交瘤开发平台：通过细胞融合筛选出稳定分泌单克隆抗体的杂交瘤细胞株，优化后的技术让抗体亲和力与特异性更高，应用于疾病诊断、免疫治疗等领域。 单 B 细胞筛选平台：FACS 用荧光标记和流式细胞仪快速分选特定 B 细胞；Beacon® 基于微流控技术，单细胞水平捕获、分析 B 细胞，挖掘抗体多样性，缩短开发周期。 凭借这些平台，艾普蒂生物为客户提供优质试剂和专业 CRO 技术服务，推动生物科技发展。
艾普蒂生物在重组蛋白和天然蛋白开发领域经验十分丰富，拥有超过 2 万种重组蛋白的开发案例。在四大重组蛋白表达平台的运用上，艾普蒂生物不仅经验老到，还积累了详实的成功案例。针对客户的工业化生产需求，我们能够定制并优化实验方案。通过小试探索、工艺放大以及条件优化等环节，对重组蛋白基因序列进行优化，全面探索多种条件，精准找出最契合客户需求的生产方法。 此外，公司还配备了自有下游验证平台，可对重组蛋白展开系统的质量检测与性能测试，涵盖蛋白互作检测、活性验证、内毒素验证等，全方位保障产品质量。 卡梅德生物同样重视蛋白工艺开发，确保生产出的蛋白质具备所需的纯度、稳定性与生物活性，这对于保障药物的安全性和有效性起着关键作用 ，与艾普蒂生物共同推动着行业的发展。
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