| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PANK3 |
| Uniprot No | Q9H999 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-370 aa |
| 活性数据 | MKIKDAKKPS FPWFGMDIGG TLVKLSYFEP IDITAEEEQE EVESLKSIRK YLTSNVAYGS TGIRDVHLEL KDLTLFGRRG NLHFIRFPTQ DLPTFIQMGR DKNFSTLQTV LCATGGGAYK FEKDFRTIGN LHLHKLDELD CLVKGLLYID SVSFNGQAEC YYFANASEPE RCQKMPFNLD DPYPLLVVNI GSGVSILAVH SKDNYKRVTG TSLGGGTFLG LCSLLTGCES FEEALEMASK GDSTQADKLV RDIYGGDYER FGLPGWAVAS SFGNMIYKEK RESVSKEDLA RATLVTITNN IGSVARMCAV NEKINRVVFV GNFLRVNTLS MKLLAYALDY WSKGQLKALF LEHEGYFGAV GALLGLPNFS |
| 分子量 | 41.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PANK3蛋白的3篇代表性文献(注：以下内容为模拟示例，实际文献需通过学术数据库检索验证)：
1. **文献名称**：*Expression, purification, and characterization of recombinant human PANK3: insights into enzyme kinetics and substrate specificity*
**作者**：Johnson A.R. et al.
**摘要**：本研究在大肠杆菌中成功表达并纯化了重组人PANK3蛋白，系统分析了其对泛酸底物的动力学参数，揭示了其与辅酶A合成相关的催化特性。
2. **文献名称**：*Structural basis of pantothenate kinase-associated neurodegeneration: Crystal structure of human PANK3*
**作者**：Smith T.L. et al.
**摘要**：通过X射线晶体学解析了PANK3的三维结构，发现其活性位点突变与神经退行性疾病的关联，为靶向药物设计提供了结构基础。
3. **文献名称**：*Functional rescue of PANK3 deficiency in cellular models using recombinant protein therapy*
**作者**：Lee J. et al.
**摘要**：体外实验表明，外源性重组PANK3蛋白可纠正细胞模型中辅酶A代谢缺陷，提示其在治疗PANK相关代谢疾病中的潜在应用。
建议通过PubMed或Google Scholar以“recombinant human PANK3”“PANK3 pantothenate kinase”等关键词检索最新文献。
Recombinant human PANK3 (Pantothenate Kinase 3) protein is a genetically engineered version of the enzyme encoded by the *PANK3* gene, which plays a critical role in coenzyme A (CoA) biosynthesis. PANK3 catalyzes the phosphorylation of pantothenate (vitamin B5) to 4’-phosphopantothenate, the first and rate-limiting step in CoA production. CoA is essential for fatty acid metabolism, energy generation, and cellular processes like protein acetylation. While humans encode four PANK isoforms (PANK1-4), PANK3 is primarily cytosolic and expressed in tissues such as the liver, kidney, and brain. Its function overlaps with other isoforms but may have tissue-specific regulatory roles.
Mutations in *PANK2* (not PANK3) are linked to pantothenate kinase-associated neurodegeneration (PKAN), a rare autosomal recessive disorder. However, studying recombinant PANK3 aids in understanding isoform-specific mechanisms and compensatory pathways. Recombinant PANK3 is produced using expression systems (e.g., *E. coli* or mammalian cells) to ensure proper folding and enzymatic activity. It serves as a tool for biochemical assays, drug screening, and elucidating CoA regulation. Researchers also explore its therapeutic potential in metabolic disorders or secondary CoA deficiencies. By enabling precise study of PANK3’s kinetics, structure, and interactions, recombinant protein technology accelerates insights into its biological and pathological significance.
×
