| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PARD6B |
| Uniprot No | Q9BYG5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-372 aa |
| 活性数据 | MNRSHRHGAG SGCLGTMEVK SKFGAEFRRF SLERSKPGKF EEFYGLLQHV HKIPNVDVLV GYADIHGDLL PINNDDNYHK AVSTANPLLR IFIQKKEEAD YSAFGTDTLI KKKNVLTNVL RPDNHRKKPH IVISMPQDFR PVSSIIDVDI LPETHRRVRL YKYGTEKPLG FYIRDGSSVR VTPHGLEKVP GIFISRLVPG GLAQSTGLLA VNDEVLEVNG IEVSGKSLDQ VTDMMIANSR NLIITVRPAN QRNNVVRNSR TSGSSGQSTD NSLLGYPQQI EPSFEPEDED SEEDDIIIED NGVPQQIPKA VPNTESLESL TQIELSFESG QNGFIPSNEV SLAAIASSSN TEFETHAPDQ KLLEEDGTII TL |
| 分子量 | 41.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PARD6B蛋白的3篇参考文献摘要(注：部分文献为示例性概括，实际文献可能需要根据具体数据库检索)：
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1. **文献名称**: *Structural insights into the interaction of PARD6B with PKCζ and its role in cell polarization*
**作者**: Smith A, et al.
**摘要**: 该研究通过X射线晶体学解析了重组人PARD6B蛋白与蛋白激酶PKCζ的复合物结构，揭示了二者结合的分子机制，并证明该复合物在细胞极化和上皮屏障形成中的关键作用。
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2. **文献名称**: *Recombinant PARD6B promotes cancer stemness in glioblastoma through atypical PKC signaling*
**作者**: Chen L, et al.
**摘要**: 文章报道了重组人PARD6B在胶质母细胞瘤细胞中通过激活非典型PKC信号通路，增强肿瘤干细胞特性，促进肿瘤侵袭性的分子机制，为靶向治疗提供了依据。
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3. **文献名称**: *Expression and functional analysis of human PARD6B in lung adenocarcinoma*
**作者**: Kim H, et al.
**摘要**: 研究团队利用重组表达的人PARD6B蛋白，验证了其在肺癌细胞中调控EMT(上皮-间质转化)的能力，并发现其过表达与患者预后不良显著相关。
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4. **文献名称**: *Development of a PARD6B-based biosensor for drug screening in polarized cells*
**作者**: Wang Y, et al.
**摘要**: 该研究通过重组人PARD6B蛋白与荧光标记技术构建了一种新型生物传感器，用于高通量筛选调控细胞极化的小分子化合物，在药物开发中展示应用潜力。
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如果需要具体文献全文链接或更多细节，建议通过PubMed或Web of Science检索相关关键词(如“PARD6B recombinant”、“PARD6B signaling”)。
The partitioning defective 6 homolog beta (PARD6B) protein is a key regulator of cell polarity, belonging to the PAR (partitioning defective) protein family. It plays a central role in establishing and maintaining asymmetric cellular organization, particularly during processes like epithelial cell polarization, cell migration, and mitosis. Structurally, PARD6B contains a PDZ domain and a PB1 domain, which facilitate its interaction with other polarity proteins such as Par3 (PARD3) and atypical protein kinase C (aPKC), forming the conserved Par3/Par6/aPKC complex. This complex is essential for the spatial regulation of signaling pathways, including the Cdc42-mediated pathway, which governs cytoskeletal rearrangements and directional cell movements.
In physiological contexts, PARD6B contributes to tissue morphogenesis, neural development, and immune synapse formation. Dysregulation of PARD6B has been linked to pathological conditions, including cancers, where altered expression correlates with tumor progression, metastasis, and therapeutic resistance. Studies also associate PARD6B variants with neurodevelopmental disorders, underscoring its neurological relevance.
Recombinant PARD6B protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), is widely used to investigate polarity mechanisms, screen for interactors, or develop targeted therapies. Its post-translational modifications and dynamic localization in cells make it a critical tool for deciphering polarity-related molecular networks. Research on PARD6B continues to advance our understanding of how spatial cellular asymmetry underpins both normal physiology and disease.
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