| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAD23B |
| Uniprot No | P54727 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-409aa |
| 氨基酸序列 | MQVTLKTLQQ QTFKIDIDPE ETVKALKEKI ESEKGKDAFP VAGQKLIYAG KILNDDTALK EYKIDEKNFV VVMVTKPKAV STPAPATTQQ SAPASTTAVT SSTTTTVAQA PTPVPALAPT STPASITPAS ATASSEPAPA SAAKQEKPAE KPAETPVATS PTATDSTSGD SSRSNLFEDA TSALVTGQSY ENMVTEIMSM GYEREQVIAA LRASFNNPDR AVEYLLMGIP GDRESQAVVD PPQAASTGAP QSSAVAAAAA TTTATTTTTS SGGHPLEFLR NQPQFQQMRQ IIQQNPSLLP ALLQQIGREN PQLLQQISQH QEHFIQMLNE PVQEAGGQGG GGGGGSGGIA EAGSGHMNYI QVTPQEKEAI ERLKALGFPE GLVIQAYFAC EKNENLAANF LLQQNFDED |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **RAD23B重组蛋白** 的模拟参考文献示例，涵盖其功能、结构及应用研究：
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1. **"Recombinant RAD23B Protein Enhances DNA Repair by Stabilizing XPC in Nucleotide Excision Repair"**
*作者：Chen, L., et al. (2016)*
**摘要**：研究报道了通过昆虫细胞系统表达重组人源RAD23B蛋白，证实其通过与XPC形成复合物增强DNA损伤识别能力，并稳定XPC免受蛋白酶体降解，从而提升核苷酸切除修复(NER)效率。
2. **"Structural Analysis of the Ubiquitin-Binding Domains of RAD23B Reveals Mechanisms of Substrate Recognition"**
*作者：Wang, Y., et al. (2018)*
**摘要**：利用X射线晶体学解析了RAD23B的泛素结合域(UBA)结构，阐明其特异性识别多泛素化底物的分子机制，为理解RAD23B在DNA修复与蛋白酶体调控中的双重功能提供结构基础。
3. **"RAD23B Overexpression Promotes Cancer Cell Survival via p53-Dependent and Independent Pathways"**
*作者：Kim, S., et al. (2019)*
**摘要**：通过重组RAD23B在肿瘤细胞中的过表达实验，发现其通过调控p53稳定性(依赖泛素连接酶MDM2)及独立于p53的DNA修复通路，增强癌细胞对化疗药物的抵抗性。
4. **"Development of a RAD23B-Dependent In Vitro Assay for Screening Proteasome Inhibitors"**
*作者：Garcia, M., et al. (2020)*
**摘要**：研究构建了基于重组RAD23B蛋白的高通量筛选系统，用于检测化合物对泛素-蛋白酶体通路的调控作用，为癌症治疗药物的开发提供新工具。
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**注**：以上文献为示例性质，实际研究中请通过学术数据库(如PubMed、Web of Science)检索并核实具体信息。RAD23B的核心研究方向包括其在DNA修复、泛素信号转导及癌症治疗中的潜在应用。
RAD23B is a multifunctional protein involved in nucleotide excision repair (NER), a critical DNA repair pathway that addresses bulky DNA lesions caused by UV radiation, chemical adducts, or oxidative damage. It belongs to the RAD23 family, sharing homology with RAD23A, and plays a dual role in both DNA repair and proteasome-mediated protein degradation. Structurally, RAD23B contains an N-terminal ubiquitin-like (UBL) domain and two ubiquitin-associated (UBA) domains, which enable interactions with the 26S proteasome and ubiquitinated substrates, linking DNA damage response to protein turnover.
In NER, RAD23B partners with XPC (xeroderma pigmentosum complementation group C) to form a stable heterodimer. This complex recognizes helix-distorting DNA lesions, initiating repair by recruiting downstream factors. RAD23B stabilizes XPC, enhances its DNA-binding affinity, and protects it from proteasomal degradation, ensuring efficient damage recognition. Beyond NER, RAD23B participates in transcription-coupled repair and may regulate apoptosis and cell cycle progression under genotoxic stress.
Recombinant RAD23B protein is typically produced in *E. coli* or mammalian expression systems for biochemical and structural studies. Its purified form enables in vitro analysis of DNA repair mechanisms, substrate interactions, and ubiquitin-dependent processes. Researchers also use it to explore RAD23B's role in diseases; mutations or dysregulation are linked to cancer susceptibility (e.g., melanoma, lung cancer) and neurodegenerative disorders like Huntington’s disease, where impaired protein quality control contributes to pathogenesis. Additionally, recombinant RAD23B serves as a tool for screening therapeutic agents targeting DNA repair pathways or proteostasis networks.
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