| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHA6 |
| Uniprot No | Q9UN73 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-950 aa |
| 活性数据 | MVFTPEDRLGKQCLLLPLLLLAAWKVGSGQLHYSVPEEAKHGTFVGRIAQDLGLELAELVPRLFRMASKDREDLLEVNLQNGILFVNSRIDREELCGRSAECSIHLEVIVDRPLQVFHVDVEVRDINDNPPLFPVEEQRVLIYESRLPDSVFPLEGASDADVGSNSILTYKLSSSEYFGLDVKINSDDNKQIGLLLKKSLDREEAPAHNLFLTATDGGKPELTGTVQLLVTVLDVNDNAPTFEQSEYEVRIFENADNGTTVIRLNASDRDEGANGAISYSFNSLVAAMVIDHFSIDRNTGEIVIRGNLDFEQENLYKILIDATDKGHPPMAGHCTVLVRILDKNDNVPEIALTSLSLPVREDAQFGTVIALISVNDLDSGANGQVNCSLTPHVPFKLVSTFKNYYSLVLDSALDRESVSAYELVVTARDGGSPSLWATASLSVEVADMNDNAPAFAQPEYTVFVKENNPPGCHIFTVSARDADAQENALVSYSLVERRVGERALSSYISVHAESGKVYALQPLDHEELELLQFQVSARDAGVPPLGSNVTLQVFVLDENDNAPALLAPRVGGTGGAVSELVPRSLGAGQVVAKVRAVDADSGYNAWLSYELQPPASSARFPFRVGLYTGEISTTRVLDEADSPRHRLLVLVKDHGEPALTATATVLVSLVESGQAPKASSRASVGAAGPEAALVDVNVYLIIAICAVSSLLVLTLLLYTALRCSAPPTEGACTADKPTLVCSSAVGSWSYSQQRRQRVCSGEGPPKMDLMAFSPSLSPCPIMMGKAENQDLNEDHDAKPRQPNPDWRYSASLRAGMHSSVHLEEAGILRAGPGGPDQQWPTVSSATPEPEAGEVSPPVGAGVNSNSWTFKYGPGNPKQSGPGELPDKFIIPGSPAIISIRQEPTNSQIDKSDFITFGKKEETKKKKKKKKGNKTQEKKEKGNSTTDNSDQ |
| 分子量 | 129.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人PCDHA6蛋白的模拟参考文献(文献信息为示例，非真实数据)：
1. **标题**: *Structural characterization of human PCDHA6 extracellular domain*
**作者**: Smith J, et al.
**摘要**: 解析了PCDHA6蛋白胞外结构域的晶体结构，揭示了其钙离子依赖性同源二聚化机制，并发现其特异性表位可能参与神经元突触黏附。
2. **标题**: *PCDHA6 promotes glioma cell invasion via Wnt/β-catenin signaling*
**作者**: Li X, Wang Y, et al.
**摘要**: 首次报道PCDHA6在胶质瘤中高表达，通过重组蛋白实验证明其激活Wnt信号通路并增强肿瘤细胞迁移能力，提示其作为癌症治疗靶点的潜力。
3. **标题**: *Recombinant PCDHA6 protein interaction network in neural development*
**作者**: Tanaka K, et al.
**摘要**: 利用重组PCDHA6蛋白进行Pull-down实验，鉴定出其在神经细胞中与MAPK通路蛋白的相互作用，表明其在神经轴突导向中的调控作用。
(注：以上文献为虚拟示例，实际研究需通过PubMed/Google Scholar检索真实文献。)
**Background of Recombinant Human PCDHA6 Protein**
Recombinant human PCDHA6 (Protocadherin Alpha 6) protein is a member of the protocadherin alpha family, a subset of calcium-dependent cell adhesion molecules within the cadherin superfamily. The PCDHA6 gene, located in the PCDHA cluster on chromosome 5q31. encodes a single-pass transmembrane protein characterized by six extracellular cadherin repeats, a transmembrane domain, and a cytoplasmic tail. Protocadherins, particularly clustered PCDHs like PCDHA6. play critical roles in neural development, including neuronal circuit formation, synaptic specificity, and dendrite self-avoidance, by mediating selective cell-cell interactions.
PCDHA6 is predominantly expressed in the central nervous system, where it contributes to establishing neural diversity and connectivity through homophilic or heterophilic binding. Dysregulation of PCDHA6 has been linked to neurodevelopmental disorders and cancer progression, potentially through altered cell adhesion, signaling, or transcriptional regulation. Recombinant PCDHA6 protein, produced via heterologous expression systems (e.g., mammalian cells or *E. coli*), retains functional domains for studying its biochemical properties, binding partners, and mechanisms in vitro. It serves as a tool for exploring neurodevelopmental pathways, disease associations, and therapeutic targeting, offering insights into the molecular basis of PCDH-mediated cellular communication.
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