| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PDS5A |
| Uniprot No | Q29RF7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-333 aa |
| 活性数据 | MIHLLAHDPDFTRSQDVDQLRDIKECLWFMLEVLMTKNENNSHAFMKKMAENIKLTRDAQSPDESKTNEKLYTVCDVALCVINSKSALCNADSPKDPVLPMKFFTQPEKDFCNDKSYISEETRVLLLTGKPKPAGVLGAVNKPLSATGRKPYVRSTGTETGSNINVNSELNPSTGNRSREQSSEAAETGVSENEENPVRIISVTPVKNIDPVKNKEINSDQATQGNISSDRGKKRTVTAAGAENIQQKTDEKVDESGPPAPSKPRRGRRPKSESQGNATKNDDLNKPINKGRKRAAVGQESPGGLEAGNAKAPKLQDLAKKAAPAERQIDLQR |
| 分子量 | 62.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PDS5A蛋白的3篇示例参考文献及摘要概括(文献为模拟示例，非真实发表论文)：
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1. **文献名称**: "PDS5A regulates sister chromatid cohesion and genome stability through direct interaction with cohesin complexes"
**作者**: Zhang et al., 2018
**摘要**: 研究揭示了PDS5A作为黏连蛋白复合体的调节因子，通过与SMC3亚基结合调控姐妹染色单体的正确分离。实验表明PDS5A缺失导致染色体分离异常和基因组不稳定性，揭示了其在有丝分裂中的关键作用。
2. **文献名称**: "Downregulation of PDS5A promotes chromosomal instability and tumorigenesis in colorectal cancer"
**作者**: Smith et al., 2019
**摘要**: 该研究发现结直肠癌中PDS5A表达显著降低，并通过体外实验证实其缺失促进染色体错误分离和微核形成。临床数据分析表明PDS5A低表达与患者预后不良相关，提示其作为潜在肿瘤抑制因子的角色。
3. **文献名称**: "Structural insights into PDS5A-mediated chromatin interactions during DNA damage repair"
**作者**: Lee et al., 2020
**摘要**: 通过冷冻电镜解析PDS5A与DNA损伤修复蛋白RAD51的复合体结构，阐明其如何协调染色质构象变化以促进同源重组修复。功能实验证明PDS5A敲除导致DNA修复效率显著下降。
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**备注**：以上为模拟文献，实际研究中建议通过PubMed、Google Scholar等平台以关键词“PDS5A”、“cohesin”、“chromosome segregation”检索真实论文。
The PDS5A (PDS5 cohesin-associated factor A) protein is a crucial component of the cohesin complex, which plays a vital role in chromosome organization, sister chromatid cohesion, and DNA repair. As a regulatory subunit, PDS5A dynamically interacts with cohesin to modulate its stability, loading, and removal from chromosomes during the cell cycle. It is essential for maintaining genomic integrity by ensuring proper segregation of chromosomes during mitosis and meiosis. Dysregulation of PDS5A has been linked to chromosomal instability disorders, developmental abnormalities, and cancers, underscoring its importance in cellular homeostasis.
Structurally, PDS5A contains multiple HEAT repeats that mediate protein-protein interactions, particularly with cohesin subunits (e.g., SMC1A, SMC3) and regulatory proteins like WAPL. Its function is fine-tuned by post-translational modifications, including phosphorylation during mitosis. Recombinant human PDS5A is typically expressed in mammalian or insect cell systems to preserve its native folding and post-translational modifications. Purified recombinant PDS5A serves as a key tool for studying cohesin dynamics, chromosome biology, and disease mechanisms. Recent studies also suggest its involvement in transcriptional regulation and 3D genome architecture, expanding its functional repertoire beyond canonical cohesion roles.
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