| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PALMD |
| Uniprot No | Q9NP74 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-551aa |
| 氨基酸序列 | MEEAELVKGR LQAITDKRKI QEEISQKRLK IEEDKLKHQH LKKKALREKW LLDGISSGKE QEEMKKQNQQ DQHQIQVLEQ SILRLEKEIQ DLEKAELQIS TKEEAILKKL KSIERTTEDI IRSVKVEREE RAEESIEDIY ANIPDLPKSY IPSRLRKEIN EEKEDDEQNR KALYAMEIKV EKDLKTGEST VLSSIPLPSD DFKGTGIKVY DDGQKSVYAV SSNHSAAYNG TDGLAPVEVE ELLRQASERN SKSPTEYHEP VYANPFYRPT TPQRETVTPG PNFQERIKIK TNGLGIGVNE SIHNMGNGLS EERGNNFNHI SPIPPVPHPR SVIQQAEEKL HTPQKRLMTP WEESNVMQDK DAPSPKPRLS PRETIFGKSE HQNSSPTCQE DEEDVRYNIV HSLPPDINDT EPVTMIFMGY QQAEDSEEDK KFLTGYDGII HAELVVIDDE EEEDEGEAEK PSYHPIAPHS QVYQPAKPTP LPRKRSEASP HENTNHKSPH KNSISLKEQE ESLGSPVHHS PFDAQTTGDG TEDPSLTALR MRMAKLGKKV I |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PALMD重组蛋白的假设性参考文献示例(仅供参考，非真实文献)：
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1. **标题**：*Recombinant PALMD promotes vascular smooth muscle cell migration via RhoA signaling*
**作者**：Smith J, et al.
**摘要**：本研究利用大肠杆菌表达系统成功制备重组人源PALMD蛋白，发现其通过激活RhoA通路增强平滑肌细胞迁移能力，提示PALMD在血管重塑中的潜在作用。
2. **标题**：*Structural characterization of PALMD recombinant protein and its calcium-binding properties*
**作者**：Zhang Y, et al.
**摘要**：通过哺乳动物细胞表达纯化PALMD重组蛋白，解析其N端结构域与钙离子结合的特性，为研究其参与细胞内钙信号调控提供依据。
3. **标题**：*PALMD overexpression in HEK293 cells alters endosomal trafficking*
**作者**：Lee S, et al.
**摘要**：构建PALMD重组表达质粒并在HEK293细胞中过表达，发现其通过调控Rab5依赖的内体运输途径影响膜蛋白分选，揭示其在细胞内膜运输中的功能。
4. **标题**：*Development of a PALMD-specific monoclonal antibody using recombinant protein immunization*
**作者**：Chen L, et al.
**摘要**：利用昆虫细胞表达系统获得高纯度PALMD重组蛋白，制备特异性单克隆抗体，验证其在多种组织中的表达分布，为疾病诊断提供工具。
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**注意**：以上文献为模拟示例，实际研究中需通过学术数据库(如PubMed、Web of Science)检索真实发表的论文。若需具体文献，建议补充PALMD研究方向(如疾病关联、分子机制等)以便精准查询。
**Background of PALMD Recombinant Protein**
PALMD (Paladin), also known as phosphatase domain-containing protein, is a ubiquitously expressed cytosolic protein implicated in various cellular processes, including cytoskeletal organization, cell motility, and intracellular signaling. It belongs to the myotubularin-related protein family, characterized by a conserved phosphatase domain, though its enzymatic activity remains under investigation. PALMD is particularly noted for its role in cardiovascular biology, where it interacts with actin-binding proteins and modulates pathways involved in vascular smooth muscle cell (VSMC) contraction, endothelial function, and angiogenesis. Dysregulation of PALMD has been linked to cardiovascular pathologies such as hypertension, atherosclerosis, and aortic aneurysms.
Recombinant PALMD protein is engineered using expression systems like *E. coli* or mammalian cells, enabling high-purity production for research. Its recombinant form retains functional domains, including the N-terminal coiled-coil region and C-terminal phosphatase-like domain, allowing studies on protein-protein interactions, enzymatic activity, and structural analysis. Researchers utilize PALMD recombinant protein to explore its regulatory mechanisms in cytoskeletal dynamics, its crosstalk with signaling pathways (e.g., RhoA/ROCK), and its potential as a therapeutic target.
Applications span *in vitro* assays (e.g., binding studies, phosphatase activity screening), antibody development, and disease modeling. Its role in vascular remodeling and inflammation underscores its relevance in drug discovery for cardiovascular and metabolic disorders. Further studies aim to clarify its substrate specificity and tissue-specific functions, positioning PALMD as a molecule of growing interest in molecular and translational medicine.
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