Recombinant Human PSPN protein

中文名： Persephin蛋白(PSPN)重组蛋白
别名： PSPN；Persephin

货号: PA1000-7886

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>95%SDS-PAGE.
种属	Human
靶点	PSPN
Uniprot No	O60542
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	61-156aa
氨基酸序列	MALSGPCQLW SLTLSVAELG LGYASEEKVI FRYCAGSCPR GARTQHGLAL ARLQGQGRAH GGPCCRPTRY TDVAFLDDRHRWQRLPQLSA AACGCGG
预测分子量	10 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于PSPN(Persephin，一种神经营养因子)重组蛋白研究的虚拟参考文献示例(仅供参考，实际文献请通过学术数据库查询)：

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1. **文献名称**: *Recombinant Persephin promotes survival of dopaminergic neurons in Parkinson's disease models*

**作者**: Lin LFH, et al.

**摘要**: 研究重组PSPN蛋白在体外和MPTP诱导的帕金森病模型中的神经保护作用，发现其通过激活RET受体信号通路减少多巴胺能神经元凋亡。

2. **文献名称**: *Expression and purification of bioactive human Persephin in E. coli*

**作者**: Zhang Y, et al.

**摘要**: 报道一种利用大肠杆菌表达系统高效制备重组人源PSPN蛋白的方法，并通过细胞实验验证其促进神经元突触生长的生物活性。

3. **文献名称**: *Persephin gene delivery ameliorates motor deficits in a transgenic ALS mouse model*

**作者**: Airaksinen MS, et al.

**摘要**: 通过腺相关病毒载体递送PSPN基因至肌萎缩侧索硬化症(ALS)模型小鼠，显著延缓运动神经元退变并改善运动功能。

4. **文献名称**: *Structural basis of Persephin-GFRα4 interaction revealed by cryo-EM*

**作者**: Uren RT, et al.

**摘要**: 利用冷冻电镜解析PSPN与其受体GFRα4的复合物三维结构，阐明其特异性结合机制及下游信号激活模式。

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**注意**：以上为模拟文献，实际研究请参考PubMed、Web of Science等平台以“Persephin”或“PSPN recombinant protein”为关键词检索。

背景信息

**Background of PSPN (Persephin) Recombinant Protein**

Persephin (PSPN), a member of the glial cell line-derived neurotrophic factor (GDNF) family, is a small secreted protein critical for neuronal survival, development, and maintenance. It binds to the GDNF family receptor α4 (GFRα4) and activates the RET receptor tyrosine kinase, triggering downstream signaling pathways such as PI3K/AKT and MAPK. PSPN is primarily expressed in the nervous system and plays roles in protecting dopaminergic neurons, motor neurons, and regulating kidney development.

Structurally, PSPN shares a conserved cysteine-knot motif with other GDNF family ligands, enabling dimerization and receptor interaction. Its recombinant form is produced via genetic engineering in bacterial (e.g., *E. coli*) or mammalian expression systems, followed by purification steps (e.g., affinity chromatography) to ensure high purity and bioactivity. Recombinant PSPN retains the ability to promote neuronal survival and differentiation *in vitro* and *in vivo*, making it a valuable tool for studying neuroprotection and degenerative diseases.

Research highlights PSPN’s therapeutic potential in Parkinson’s disease, Alzheimer’s disease, and spinal cord injuries. It also shows non-neuronal functions, including influencing kidney morphogenesis and metabolic regulation. Challenges include optimizing delivery methods and understanding its tissue-specific effects. Overall, PSPN recombinant protein serves as both a research reagent and a candidate for developing neuroregenerative therapies.