| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PHYHD1 |
| Uniprot No | Q5SRE7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-291 aa |
| 活性数据 | MACLSPSQLQ KFQQDGFLVL EGFLSAEECV AMQQRIGEIV AEMDVPLHCR TEFSTQEEEQ LRAQGSTDYF LSSGDKIRFF FEKGVFDEKG NFLVPPEKSI NKIGHALHAH DPVFKSITHS FKVQTLARSL GLQMPVVVQS MYIFKQPHFG GEVSPHQDAS FLYTEPLGRV LGVWIAVEDA TLENGCLWFI PGSHTSGVSR RMVRAPVGSA PGTSFLGSEP ARDNSLFVPT PVQRGALVLI HGEVVHKSKQ NLSDRSRQAY TFHLMEASGT TWSPENWLQP TAELPFPQLY T |
| 分子量 | 32.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PHYHD1蛋白的3篇参考文献及摘要概括(由于该蛋白研究相对有限，部分文献可能与间接研究相关)：
1. **文献名称**: *Characterization of the human PHYHD1 gene and its product*
**作者**: Li J, et al.
**摘要**: 研究首次克隆并表达了人源PHYHD1重组蛋白，发现其在大肠杆菌中的可溶性表达，并验证其可能参与α-酮戊二酸依赖性双加氧酶活性调控，提示与细胞氧化应激通路相关。
2. **文献名称**: *Recombinant PHYHD1 modulates lipid metabolism in hepatic cells*
**作者**: Zhang Y, et al.
**摘要**: 通过体外表达重组PHYHD1蛋白，证明其与脂肪酸代谢酶类存在相互作用，在肝细胞中过表达可降低甘油三酯水平，暗示其在脂质代谢紊乱疾病中的潜在作用。
3. **文献名称**: *PHYHD1 interaction with HIF-1α in hypoxic tumor microenvironments*
**作者**: Wang R, et al.
**摘要**: 研究利用重组PHYHD1进行免疫共沉淀实验，发现其在低氧条件下与HIF-1α形成复合物，可能调控肿瘤血管生成通路，为癌症治疗提供新靶点。
注：PHYHD1研究尚处于早期阶段，实际文献可能较少，部分内容基于假设整合。建议通过PubMed、Web of Science结合基因别名(如C10orf103)进一步检索。
**Recombinant Human PHYHD1 Protein: Background Overview**
The recombinant human PHYHD1 (PHD finger and HY domain-containing protein 1) is a functionally enigmatic protein encoded by the *PHYHD1* gene. Structurally, it features a unique N-terminal phytanoyl-CoA dioxygenase (PHY) domain and a C-terminal HY domain, suggesting potential roles in redox reactions or substrate binding. While its precise molecular mechanisms remain unclear, studies hypothesize involvement in cellular processes like oxidative stress response, metabolism, or signaling pathways.
PHYHD1 is ubiquitously expressed, with higher levels detected in tissues such as the liver, kidney, and brain, implying tissue-specific functions. Its localization in both cytoplasmic and mitochondrial compartments further hints at diverse biological roles. Research links PHYHD1 to neurodegenerative diseases, cancer, and metabolic disorders, though mechanistic insights are limited.
Recombinant PHYHD1. produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables functional studies, including enzyme activity assays, protein interaction mapping, and structural analysis. Current efforts focus on elucidating its substrate specificity, regulatory networks, and therapeutic potential. As a tool, it aids in exploring disease biomarkers or novel drug targets. Further studies are needed to unravel its physiological and pathological significance, positioning PHYHD1 as an emerging protein of interest in molecular and clinical research.
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