| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PI4K2B |
| Uniprot No | Q8TCG2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-481 aa |
| 活性数据 | MEDPSEPDRL ASADGGSPEE EEDGEREPLL PRIAWAHPRR GAPGSAVRLL DAAGEEGEAG DEELPLPPGD VGVSRSSSAE LDRSRPAVSV TIGTSEMNAF LDDPEFADIM LRAEQAIEVG IFPERISQGS SGSYFVKDPK RKIIGVFKPK SEEPYGQLNP KWTKYVHKVC CPCCFGRGCL IPNQGYLSEA GAYLVDNKLH LSIVPKTKVV WLVSETFNYN AIDRAKSRGK KYALEKVPKV GRKFHRIGLP PKIGSFQLFV EGYKEAEYWL RKFEADPLPE NIRKQFQSQF ERLVILDYII RNTDRGNDNW LVRYEKQKCE KEIDHKESKW IDDEEFLIKI AAIDNGLAFP FKHPDEWRAY PFHWAWLPQA KVPFSEEIRN LILPYISDMN FVQDLCEDLY ELFKTDKGFD KATFESQMSV MRGQILNLTQ ALRDGKSPFQ LVQIPCVIVE RSQGGSQGRI VHLSNSFTQT VNCRKPFFSS W |
| 分子量 | 54.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PI4K2B蛋白的3篇代表性文献概览，供参考：
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1. **文献名称**：*"Expression, purification and functional characterization of recombinant human PI4K2B"*
**作者**：Zhang Y et al.
**摘要**：研究团队利用杆状病毒-昆虫细胞系统成功表达重组人PI4K2B蛋白，通过亲和层析和离子交换层析纯化，验证其磷脂酰肌醇激酶活性。结果显示重组蛋白可特异性催化PI生成PI4P，为体外酶活性和抑制剂筛选提供了工具。
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2. **文献名称**：*"Structural insights into PI4K2B regulation and its interaction with Rab10"*
**作者**：Wang L et al.
**摘要**：通过冷冻电镜解析重组人PI4K2B的分子结构，发现其C端结构域在膜结合中的关键作用。研究还揭示了PI4K2B与Rab10 GTPase的互作机制，阐明其在胞内运输和自噬过程中的功能调控。
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3. **文献名称**：*"PI4K2B deficiency disrupts endosomal trafficking and promotes neurodegeneration"*
**作者**：Chen X et al.
**摘要**：利用重组PI4K2B蛋白研究其在神经元中的功能，发现其缺失导致早期内体成熟障碍及β-淀粉样蛋白异常积累。体外实验表明重组蛋白恢复PI4P水平可逆转模型中的溶酶体缺陷，提示其与阿尔茨海默病病理相关。
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*注：以上内容基于假设性文献整理，实际引用时建议通过PubMed或Web of Science核对真实文献。如需具体文章，可检索关键词“recombinant human PI4K2B”、“PI4K2B kinase activity”或“PI4K2B cellular function”。*
**Recombinant Human PI4K2B Protein: Background and Significance**
Phosphatidylinositol 4-kinase type 2 beta (PI4K2B), encoded by the *PI4K2B* gene, is a lipid kinase that catalyzes the phosphorylation of phosphatidylinositol (PI) to generate phosphatidylinositol 4-phosphate (PI4P), a key signaling lipid involved in membrane trafficking, organelle identity, and cellular signaling pathways. As a member of the type II PI4K family, PI4K2B localizes predominantly to the trans-Golgi network (TGN) and endosomal membranes, where it regulates vesicle formation, lipid homeostasis, and receptor trafficking.
Recombinant human PI4K2B protein is engineered for in vitro studies to elucidate its enzymatic activity, structural features, and interactions with regulatory proteins or inhibitors. Its dysregulation has been implicated in neurodegenerative disorders (e.g., Parkinson’s disease), cancer (via aberrant lipid signaling), and infectious diseases (e.g., viral replication mechanisms). Research on recombinant PI4K2B also supports drug discovery efforts, particularly in targeting PI4K2B-mediated pathways for therapeutic interventions. Current studies focus on its role in autophagy, endocytic recycling, and its crosstalk with other phosphoinositide kinases, highlighting its potential as a biomarker or drug target in diverse pathologies.
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