| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PITX2 |
| Uniprot No | Q99697 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-317 aa |
| 活性数据 | METNCRKLVS ACVQLGVQPA AVECLFSKDS EIKKVEFTDS PESRKEAASS KFFPRQHPGA NEKDKSQQGK NEDVGAEDPS KKKRQRRQRT HFTSQQLQEL EATFQRNRYP DMSTREEIAV WTNLTEARVR VWFKNRRAKW RKRERNQQAE LCKNGFGPQF NGLMQPYDDM YPGYSYNNWA AKGLTSASLS TKSFPFFNSM NVNPLSSQSM FSPPNSISSM SMSSSMVPSA VTGVPGSSLN SLNNLNNLSS PSLNSAVPTP ACPYAPPTPP YVYRDTCNSS LASLRLKAKQ HSSFGYASVQ NPASNLSACQ YAVDRPV |
| 分子量 | 35.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人PITX2蛋白的代表性文献示例(文献信息为模拟概括，仅供参考)：
1. **文献名称**：*PITX2 regulates left-right cardiac development through Wnt/β-catenin signaling*
**作者**：Smith A, et al.
**摘要**：研究通过重组人PITX2蛋白体外实验，揭示其在胚胎心脏左右不对称发育中调控Wnt/β-catenin通路，影响心肌细胞定向分化的分子机制。
2. **文献名称**：*PITX2 overexpression promotes cell proliferation in breast cancer via direct transcriptional activation of CCND1*
**作者**：Chen L, et al.
**摘要**：利用重组PITX2蛋白及基因编辑技术，证明其通过结合细胞周期蛋白CCND1启动子区，促进乳腺癌细胞异常增殖，提供新的癌症治疗靶点证据。
3. **文献名称**：*Recombinant PITX2 protein enhances osteogenic differentiation of mesenchymal stem cells in vitro*
**作者**：Kimura T, et al.
**摘要**：报道重组PITX2蛋白通过调控BMP2/Smad通路，显著增强间充质干细胞的成骨分化能力，为骨组织再生提供了潜在应用方向。
注：上述文献为科研场景模拟案例，实际引用需查询具体数据库(如PubMed、Web of Science)获取真实论文。
PITX2 (Paired-like Homeodomain Transcription Factor 2) is a member of the bicoid-like homeodomain transcription factor family, playing crucial roles in embryonic development, particularly in organogenesis of the heart, eyes, teeth, and pituitary gland. It regulates asymmetric left-right patterning during embryogenesis by controlling downstream target genes. Mutations in the human *PITX2* gene are linked to Axenfeld-Rieger syndrome, characterized by ocular, dental, and craniofacial abnormalities, as well as congenital heart defects. The protein contains a conserved homeodomain for DNA binding and transcriptional activation, along with C-terminal regions that mediate protein-protein interactions. Recombinant human PITX2 protein is typically produced using bacterial (e.g., *E. coli*) or mammalian expression systems to ensure proper folding and post-translational modifications. Purified PITX2 is used in studies exploring its regulatory mechanisms in developmental pathways, its interaction with signaling molecules like β-catenin, and its role in disease pathogenesis. Researchers also employ it to develop cellular models for drug screening or gene therapy strategies targeting PITX2-related disorders. Its recombinant form often includes affinity tags (e.g., His-tag) for easier isolation and detection in experimental assays.
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