| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLEKHA8 |
| Uniprot No | Q96JA3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-519 aa |
| 活性数据 | MEGVLYKWTN YLSGWQPRWF LLCGGILSYY DSPEDAWKGC KGSIQMAVCE IQVHSVDNTR MDLIIPGEQY FYLKARSVAE RQRWLVALGS AKACLTDSRT QKEKEFAENT ENLKTKMSEL RLYCDLLVQQ VDKTKEVTTT GVSNSEEGID VGTLLKSTCN TFLKTLEECM QIANAAFTSE LLYRTPPGSP QLAMLKSSKM KHPIIPIHNS LERQMELSTC ENGSLNMEIN GEEEILMKNK NSLYLKSAEI DCSISSEENT DDNITVQGEI RKEDGMENLK NHDNNLTQSG SDSSCSPECL WEEGKEVIPT FFSTMNTSFS DIELLEDSGI PTEAFLASCY AVVPVLDKLG PTVFAPVKMD LVGNIKKVNQ KYITNKEEFT TLQKIVLHEV EADVAQVRNS ATEALLWLKR GLKFLKGFLT EVKNGEKDIQ TALNNAYGKT LRQHHGWVVR GVFALALRAA PSYEDFVAAL TVKEGDHQKE AFSIGMQRDL SLYLPAMEKQ LAILDTLYEV HGLESDEVV |
| 分子量 | 58.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于研究背景模拟的关于重组人PLEKHA8蛋白的参考文献示例(注：文献为虚构，供参考使用)：
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1. **文献名称**: "Recombinant Expression and Functional Analysis of Human PLEKHA8 in Vesicular Trafficking"
**作者**: Johnson R., et al.
**摘要**: 本研究利用昆虫细胞系统成功表达并纯化重组人PLEKHA8蛋白，揭示了其PH结构域与细胞内膜转运囊泡的相互作用，表明PLEKHA8可能在胞内运输过程中调控蛋白质分选。
2. **文献名称**: "Structural Characterization of PLEKHA8 and Its Role in Maintaining Epithelial Cell Junctions"
**作者**: Chen L., et al.
**摘要**: 通过重组人PLEKHA8蛋白的晶体结构解析，发现其C端结构域与极性蛋白Par3的结合能力，提示PLEKHA8在上皮细胞紧密连接形成及维持中的关键作用。
3. **文献名称**: "PLEKHA8 Modulates EGFR Signaling via Phosphoinositide Binding: Insights from Recombinant Protein Studies"
**作者**: Müller S., et al.
**摘要**: 本文报道重组PLEKHA8蛋白特异性结合PI(4.5)P2脂质，并通过调控EGFR内吞影响下游信号通路，为癌症靶向治疗提供潜在靶点。
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**建议**：实际文献检索可通过PubMed或Google Scholar等平台，使用关键词“recombinant PLEKHA8”“PLEKHA8 function”或结合疾病关键词(如“cancer”“cell polarity”)进行拓展。若研究较少，可关注PH结构域家族或细胞极性相关综述中的引用。请注意核对蛋白名称拼写(如是否存在同源蛋白PLEKHA7或PLEKHA6混淆)。
PLEKHA8 (Pleckstrin Homology Domain-Containing Protein A8) is a member of the pleckstrin homology (PH) domain-containing protein family, which plays diverse roles in intracellular signaling, membrane trafficking, and cytoskeletal organization. The human PLEKHA8 gene encodes a protein of approximately 1.300 amino acids, characterized by an N-terminal PH domain critical for phosphoinositide binding and subcellular targeting. It is predominantly localized to the trans-Golgi network (TGN), where it regulates vesicular transport, cargo sorting, and maintenance of Golgi structure.
Studies suggest PLEKHA8 interacts with components of the exocyst complex and Rab GTPases, implicating it in polarized exocytosis and cell polarity. Its PH domain binds phosphatidylinositol (PI) lipids like PI(4.5)P2 or PI(3)P, linking membrane dynamics to signaling pathways. Dysregulation of PLEKHA8 has been associated with cancer progression, particularly in enhancing cell proliferation and metastasis through unclear mechanisms. Recombinant human PLEKHA8 protein is often expressed in mammalian or insect cell systems to preserve post-translational modifications, enabling functional studies on its role in Golgi-vesicle trafficking, autophagy, and disease models. Recent research also highlights potential involvement in metabolic disorders, as PLEKHA8 knockout mice display impaired insulin secretion. Despite growing interest, its precise molecular interactions and therapeutic relevance remain under exploration.
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