| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLEC1 |
| Uniprot No | Q15149 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 4384-4493 aa |
| 活性数据 | CGFEDPRTKTKMSAAQALKKGWLYYEAGQRFLEVQYLTGGLIEPDTPGRVPLDEALQRGTVDARTAQKLRDVGAYSKYLTCPKTKLKISYKDALDRSMVEEGTGLRLLEA |
| 分子量 | 37.84 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人PLEC1(plectin)蛋白相关的参考文献示例(文献标题和作者为虚构示例，供参考格式)：
1. **标题**: *Production and functional analysis of recombinant human plectin in keratinocytes*
**作者**: Winter L., et al.
**摘要**: 研究通过哺乳动物表达系统成功表达重组PLEC1蛋白，证实其在维持角质细胞骨架稳定性中的作用，并发现其与整合素β4的相互作用增强细胞黏附。
2. **标题**: *Plectin isoforms reconstitute cytoskeletal defects in epidermolysis bullosa models*
**作者**: Pfisterer S., et al.
**摘要**: 利用重组PLEC1蛋白恢复大疱性表皮松解症患者来源细胞的细胞骨架结构，证明其可通过调节中间丝网络修复机械应激缺陷。
3. **标题**: *Structural mapping of plectin's intermediate filament binding domain*
**作者**: Andrä K., et al.
**摘要**: 通过重组表达PLEC1的特定结构域并进行晶体学分析，揭示了plectin与波形蛋白(vimentin)结合的分子机制。
4. **标题**: *Recombinant plectin as a therapeutic agent for muscular dystrophy*
**作者**: Rezniczek G.A., et al.
**摘要**: 在小鼠模型中评估重组PLEC1蛋白的疗效，显示其改善肌纤维膜稳定性并延缓肌营养不良症的病理进展。
(注：以上文献信息为示例模型生成，实际引用需根据真实文献调整。)
Plectin, encoded by the *PLEC1* gene, is a large cytoskeletal linker protein critical for maintaining cellular integrity and mechanical stability. Functioning as a versatile scaffold, it interacts with multiple cytoskeletal components, including actin, microtubules, and intermediate filaments, facilitating their organization and cross-linking. Plectin also anchors cytoskeletal structures to membrane-associated complexes, such as hemidesmosomes in epithelial cells, ensuring tissue cohesion under mechanical stress. Beyond structural roles, it participates in signaling pathways, organelle positioning, and cell migration. Mutations in *PLEC1* are linked to severe disorders like epidermolysis bullosa simplex (EBS) and muscular dystrophy, highlighting its physiological importance.
Recombinant human PLEC1 protein, produced via heterologous expression systems (e.g., bacteria, mammalian cells), enables detailed mechanistic studies of its multifunctional domains. Researchers utilize it to investigate binding partners, structural dynamics, and disease-related mutations. Its applications extend to developing therapeutic strategies, such as gene therapy or small-molecule interventions targeting plectin dysfunction. Despite challenges in producing full-length plectin due to its size (~500 kDa), truncated recombinant variants focusing on specific domains (e.g., actin-binding or intermediate filament-binding regions) remain invaluable for dissecting its roles in cellular homeostasis and disease pathology. This protein continues to be a focal point in understanding cytoskeletal regulation and related pathologies.
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